Dall'Olio Adriano Jaskonis, Matias Gustavo de Sá Schiavo, Carreira Ana Claudia Oliveira, de Carvalho Hianka Jasmyne Costa, van den Broek Campanelli Thais, da Silva Thamires Santos, da Silva Mônica Duarte, Abreu-Silva Ana Lúcia, Miglino Maria Angélica
Surgery Department, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo 05508-270, Brazil.
Department of Veterinary Pathology, State University of Maranhão, Maranhão 65055-150, Brazil.
Materials (Basel). 2022 Sep 1;15(17):6027. doi: 10.3390/ma15176027.
Complex wounds in dogs are a recurrent problem in veterinary clinical application and can compromise skin healing; in this sense, tissue bioengineering focused on regenerative medicine can be a great ally. Decellularized and recellularized skin scaffolds are produced to be applied in different and complex canine dermal wounds in the present investigation. Dog skin fragments are immersed in a 0.5% sodium dodecyl sulfate (SDS) solution at room temperature and overnight at 4 °C for 12 days. Decellularized samples are evaluated by histological analysis, scanning electron microscopy (SEM) and gDNA quantification. Some fragments are also recellularized using mesenchymal stem cells (MSCs). Eight adult dogs are divided into three groups for the application of the decellularized (Group I, = 3) and recellularized scaffolds (Group II, = 3) on injured areas, and a control group (Group III, = 2). Wounds are evaluated and measured during healing, and comparisons among the three groups are described. In 30- and 60-day post-grafting, the histopathological analysis of patients from Groups I and II shows similar patterns, tissue architecture preservation, epithelial hyperplasia, hyperkeratosis, edema, and mononuclear inflammatory infiltrate. Perfect integration between scaffolds and wounds, without rejection or contamination, are observed in both treated groups. According to these results, decellularized skin grafts may constitute a potential innovative and functional tool to be adopted as a promising dog cutaneous wound treatment. This is the first study that applies decellularized and recellularized biological skin grafts to improve the healing process in several complex wounds in dogs, demonstrating great potential for regenerative veterinary medicine progress.
犬类复杂伤口是兽医临床应用中反复出现的问题，会影响皮肤愈合；从这个意义上说，专注于再生医学的组织生物工程可能是一个很好的助力。在本研究中，制备了去细胞化和再细胞化的皮肤支架，用于不同的复杂犬类皮肤伤口。将犬类皮肤碎片在室温下浸入0.5%的十二烷基硫酸钠（SDS）溶液中，并在4℃下过夜浸泡12天。通过组织学分析、扫描电子显微镜（SEM）和基因组DNA定量对去细胞化样本进行评估。一些碎片也使用间充质干细胞（MSCs）进行再细胞化。将8只成年犬分为三组，分别在受伤部位应用去细胞化支架（第一组，n = 3）和再细胞化支架（第二组，n = 3），以及一个对照组（第三组，n = 2）。在伤口愈合过程中对其进行评估和测量，并描述三组之间的比较情况。在移植后30天和60天，对第一组和第二组患者的组织病理学分析显示出相似的模式，包括组织结构保留、上皮增生、角化过度、水肿和单核炎性浸润。在两个治疗组中均观察到支架与伤口完美整合，无排斥或污染现象。根据这些结果，去细胞化皮肤移植物可能构成一种潜在的创新和功能性工具，有望成为犬类皮肤伤口治疗的有效方法。这是第一项应用去细胞化和再细胞化生物皮肤移植物来改善犬类多种复杂伤口愈合过程的研究，证明了再生兽医学发展的巨大潜力。
