School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China; Institute of Environmental Biology and Life Support Technology, Beihang University, Beijing 100191, China.
School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China; Institute of Environmental Biology and Life Support Technology, Beihang University, Beijing 100191, China; Beijing Advanced Innovation Centre for Biomedical Engineering, Beihang University, Beijing 100083, China.
Life Sci Space Res (Amst). 2020 May;25:136-142. doi: 10.1016/j.lssr.2019.10.003. Epub 2019 Oct 16.
Bioregenerative Life Support System (BLSS) is a closed artificial ecosystem and could provide oxygen, food, water and other substances for space survival. Solid waste treatment is a key rate-limiting step in BLSS. In this study, solid wastes including wheat straw, human and yellow mealworm feces were disposed in a semi-continuous bio-convertor for 105 days in a ground-based experimental BLSS platform (Lunar Palace 1). Solid wastes at different periods were sampled and the microbial community variation, functional genes and metabolic pathways were analyzed. The results showed phyla Firmicutes, Bacteroidetes and Proteobacteria predominated in all samples. While microbial community structures at genus level were significantly different, indicating selective enrichment during the 105-day process. The abundance of functional gene related to carbohydrate transport and metabolism was predicted higher on 45-day and 70-day. The metabolic pathway analysis revealed the degradation mechanisms and provided evidence for metabolic regulation.
生物再生生命保障系统（BLSS）是一个封闭的人工生态系统，可以为太空生存提供氧气、食物、水和其他物质。固体废物处理是 BLSS 的关键限速步骤。在这项研究中，将包括小麦秸秆、人和黄粉虫粪便在内的固体废物在基于地面的实验 BLSS 平台（月宫一号）中连续半连续生物转化器中处理了 105 天。对不同时期的固体废物进行了采样，并分析了微生物群落的变化、功能基因和代谢途径。结果表明，所有样品中优势菌门为厚壁菌门、拟杆菌门和变形菌门。而在属水平上微生物群落结构有显著差异，表明在 105 天的过程中存在选择性富集。预测与碳水化合物运输和代谢相关的功能基因的丰度在 45 天和 70 天更高。代谢途径分析揭示了降解机制，并为代谢调控提供了证据。
