高通量 DNA 序列分析 AMF 群落。

High-Throughput DNA Sequence-Based Analysis of AMF Communities.

机构信息

Institute for Sustainable Plant Protection, National Research Council (CNR), Torino, Italy.

Department of Life Sciences and Systems Biology, University of Turin, Torino, Italy.

出版信息

Methods Mol Biol. 2020;2146:99-116. doi: 10.1007/978-1-0716-0603-2_9.

DOI:10.1007/978-1-0716-0603-2_9

PMID:32415599

Abstract

Arbuscular mycorrhizal fungi (AMF) are obligate symbionts of most land plants. They have great ecological and economic impacts as they support plant nutrition and water supply, soil structure, and plant resistance to pathogens. Investigating AMF presence and distribution at small and large scales is critical. Therefore, research requires standard protocols to be easily implemented. In this chapter, we describe a workflow for AMF identification by high-throughput sequencing through Illumina MiSeq platform of two DNA target regions: small subunit (SSU) and internal transcribed spacer (ITS). The protocol can apply to both soil and root AMF communities.

摘要

丛枝菌根真菌（AMF）是大多数陆地植物的专性共生体。它们具有巨大的生态和经济影响，因为它们支持植物营养和水分供应、土壤结构以及植物对病原体的抗性。在小尺度和大尺度上调查 AMF 的存在和分布至关重要。因此，研究需要易于实施的标准协议。在本章中，我们描述了通过 Illumina MiSeq 平台对两个 DNA 靶区域（小亚基 (SSU) 和内部转录间隔区 (ITS)）进行高通量测序来鉴定 AMF 的工作流程。该方案可适用于土壤和根 AMF 群落。