Stanimirović D, Djuricić B M, Mrsulja B B
Institute of Biochemistry, School of Medicine, Belgrade, Yugoslavia.
Metab Brain Dis. 1988 Dec;3(4):293-6. doi: 10.1007/BF00999540.
The activity of glutathione reductase (GR) was measured in crude mitochondrial fraction isolated from cerebral cortex and basal ganglia of Mongolian gerbils subjected to bilateral carotid occlusion of various duration (1, 2, 3, 5, 10, and 15 min), or reflow (1, 24, and 96 hr) following ischemia (5 or 15 min). Ischemia up to 5 min does not induce changes in GR activity in either structure. Basal ganglia activity is halved at 10 min and cortical at 15 min of ischemia. In reflow, basal ganglia GR activity is diminished, while cortical GR is transiently reduced at day 1 of reflow. The persistent and profound decrease in GR activity in basal ganglia following ischemia is indicative of the lowered antioxidative capacity of these cells, being possibly related to their greater vulnerability toward ischemia.
在蒙古沙鼠大脑皮层和基底神经节分离出的粗线粒体部分中，测定了谷胱甘肽还原酶（GR）的活性。这些沙鼠经历了不同时长（1、2、3、5、10和15分钟）的双侧颈动脉闭塞，或在缺血（5或15分钟）后进行再灌注（1、24和96小时）。长达5分钟的缺血不会在任一结构中诱导GR活性的变化。缺血10分钟时基底神经节的活性减半，缺血15分钟时皮层的活性减半。在再灌注过程中，基底神经节的GR活性降低，而皮层的GR在再灌注第1天短暂降低。缺血后基底神经节中GR活性持续且显著降低，表明这些细胞的抗氧化能力降低，这可能与它们对缺血的更大易损性有关。
