Vander Straeten Aurélien, Lefèvre Damien, Demoustier-Champagne Sophie, Dupont-Gillain Christine
Institute of Condensed Matter and Nanosciences, Université catholique de Louvain, Place Louis Pasteur, 1 bte L4.01.10, B-1348 Louvain-la-Neuve, Belgium.
Institute of Condensed Matter and Nanosciences, Université catholique de Louvain, Place Louis Pasteur, 1 bte L4.01.10, B-1348 Louvain-la-Neuve, Belgium.
Adv Colloid Interface Sci. 2020 Jun;280:102161. doi: 10.1016/j.cis.2020.102161. Epub 2020 Apr 19.
The immobilization of proteins to impart specific functions to surfaces is topical for chemical engineering, healthcare and diagnosis. Layer-by-Layer (LbL) self-assembly is one of the most used method to immobilize macromolecules on surfaces. It consists in the alternate adsorption of oppositely charged species, resulting in the formation of a multilayer. This method in principle allows any charged object to be immobilized on any surface, from aqueous solutions. However, when it comes to proteins, the promises of versatility, simplicity and universality that the LbL approach holds are unmet due to the heterogeneity of protein properties. In this review, the literature is analyzed to make a generic approach emerge, with a view to facilitate the LbL assembly of proteins with polyelectrolytes (PEs). In particular, this review aims at guiding the choice of the PE and the building conditions that lead to the successful growth of protein-based multilayered self-assemblies.
将蛋白质固定于表面以赋予其特定功能，这一课题在化学工程、医疗保健及诊断领域备受关注。层层（LbL）自组装是将大分子固定于表面最常用的方法之一。它是通过带相反电荷的物质交替吸附来实现的，从而形成多层结构。原则上，这种方法允许任何带电物体从水溶液中固定于任何表面。然而，当涉及到蛋白质时，由于蛋白质性质的异质性，层层法所具有的多功能性、简单性和通用性的优势并未得到充分体现。在本综述中，对相关文献进行了分析，以期形成一种通用方法，以促进蛋白质与聚电解质（PEs）的层层组装。特别是，本综述旨在指导聚电解质的选择以及构建条件的确定，从而实现基于蛋白质的多层自组装的成功生长。
