Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748, Olsztyn, Poland.
Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748, Olsztyn, Poland.
Theriogenology. 2020 Sep 1;153:1-8. doi: 10.1016/j.theriogenology.2020.04.039. Epub 2020 May 6.
Prostaglandins (PGs) play crucial roles in the regulation of the oestrus cycle and establishment of pregnancy in animals. Luteinizing hormone (LH) and ovarian steroids are involved in regulating endometrial PG production in many species. Their effects on PG production and associated pathways in the mare myometrium and endometrium are the subjects of our interest. This study aimed to evaluate the specific effects of LH and ovarian steroids on equine myometrial and endometrial tissues on (i) PGE and PGF secretion and (ii) transcription of genes encoding specific enzymes responsible for PG synthesis, such as prostaglandin-endoperoxide synthase (PTGS2), PGE synthases (PGES), PGF synthases (PGFS), and PGI synthases (PGIS), using equine myometrial and endometrial explants. Equine myometrial and endometrial tissues were collected at the mid-luteal (n = 6) and follicular (n = 6) phases of the oestrus cycle and were exposed to: (1) vehicle (control), (2) arachidonic acid (AA, 50 ng/mL, positive control), (3) LH (10 ng/mL), (4) progesterone (P, 10M) and (5) 17-β oestradiol (E, 10M) for 24 h. After exposure, PGF and PGE concentrations were determined using direct enzyme immunoassays. Alterations in PG synthase mRNA expression were determined using RT-qPCR. After 24 h, LH and P increased PGE and PGF secretion by myometrial tissues at the mid-luteal phase (P < 0.05), whereas PG secretion was augmented by LH and E during the follicular phase (P < 0.01). In contrast, LH and E increased PGE and PGF secretion by endometrial tissues during the mid-luteal phase (P < 0.05), while E enhanced PGE secretion during the follicular phase of the oestrus cycle (P < 0.01). These results indicate that LH and ovarian steroids modulate PG production in equine myometrial and endometrial tissues and affect PG synthase expression at the mRNA level. We conclude that the equine myometrium is an alternative source of PG production and participates in the regulation of uterus function during the oestrus cycle.
前列腺素（PGs）在动物发情周期的调节和妊娠建立中起着至关重要的作用。促黄体激素（LH）和卵巢类固醇参与调节许多物种的子宫内膜 PG 产生。它们对母马子宫肌层和子宫内膜中 PG 产生的影响及其相关途径是我们感兴趣的主题。本研究旨在评估 LH 和卵巢类固醇对发情周期中期（n=6）和卵泡期（n=6）马子宫和子宫内膜组织的具体影响，即：（i）PGE 和 PGF 的分泌；（ii）编码特定 PG 合成酶的基因的转录，如前列腺素内过氧化物合酶（PTGS2）、PGE 合酶（PGES）、PGF 合酶（PGFS）和 PGI 合酶（PGIS），使用马子宫和子宫内膜组织外植体。马子宫和子宫内膜组织在发情周期的中期和卵泡期分别采集，并暴露于：（1）载体（对照）、（2）花生四烯酸（AA，50ng/mL，阳性对照）、（3）LH（10ng/mL）、（4）孕酮（P，10M）和（5）17-β 雌二醇（E，10M）24 小时。暴露后，使用直接酶免疫测定法测定 PGF 和 PGE 浓度。使用 RT-qPCR 测定 PG 合酶 mRNA 表达的变化。24 小时后，LH 和 P 在发情周期中期增加了子宫肌层组织的 PGE 和 PGF 分泌（P<0.05），而 LH 和 E 在卵泡期增加了 PG 分泌（P<0.01）。相反，LH 和 E 在发情周期中期增加了子宫内膜组织的 PGE 和 PGF 分泌（P<0.05），而 E 在发情周期的卵泡期增加了 PGE 分泌（P<0.01）。这些结果表明，LH 和卵巢类固醇调节马子宫和子宫内膜组织中的 PG 产生，并影响 PG 合酶在 mRNA 水平上的表达。我们得出结论，马子宫肌是 PG 产生的替代来源，并参与发情周期中子宫功能的调节。
