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长链非编码 RNA DCST1-AS1 通过甲基化下调胶质母细胞瘤（GBM）中的 miR-29b 促进癌细胞增殖。

LncRNA DCST1-AS1 downregulates miR-29b through methylation in glioblastoma (GBM) to promote cancer cell proliferation.

机构信息

Department of NeurosurgeryHuangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Huangshi City, 435000, Hubei Province, People's Republic of China.

Department of Rehabilitation, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, No. 141 Tianjin Road, Huangshi Gang District, Huangshi City, 435000, Hubei Province, People's Republic of China.

出版信息

Clin Transl Oncol. 2020 Dec;22(12):2230-2235. doi: 10.1007/s12094-020-02363-1. Epub 2020 May 16.

DOI:10.1007/s12094-020-02363-1
PMID:32418155
Abstract

INTRODUCTION

The role of DCST1-AS1 has been investigated in several types of cancer, while the role of DCST1-AS1 in glioblastoma (GBM) is unclear. This study aimed to investigate the role of DCST1-AS1 in GBM.

METHODS

GBM and paired non-tumor tissues were collected from 62 GBM patients. Expression levels of DCST1-AS1 and miR-29b in paired tissue samples were determined by RT-qPCR. The role of DCST1-AS1 in regulating the methylation of miR-29b was assessed by methylation-specific PCR (MSP). Cell proliferation was analyzed by cell proliferation assay.

RESULTS

It was observed that the upregulation of DCST1-AS1 in GBM predicted poor survival. MiR-29b was downregulated in GBM and inversely correlated with the expression of DCST1-AS1. In GBM cells, overexpression of DCST1-AS1 resulted in the downregulation of miR-29b and the increased methylation level of miR-29b gene. Overexpression of DCST1-AS1 resulted in increased cell proliferation. Moreover, Overexpression of DCST1-AS1 significantly reversed the inhibitory effects of miR-29b on cancer cell proliferation.

CONCLUSION

DCST1-AS1 may downregulate miR-29b through methylation in GBM to promote cancer cell proliferation.

摘要

简介

DCST1-AS1 在几种类型的癌症中作用已被研究，而其在胶质母细胞瘤（GBM）中的作用尚不清楚。本研究旨在探讨 DCST1-AS1 在 GBM 中的作用。

方法

收集 62 例 GBM 患者的 GBM 组织及配对的非肿瘤组织。采用 RT-qPCR 检测配对组织样本中 DCST1-AS1 和 miR-29b 的表达水平。通过甲基化特异性 PCR（MSP）评估 DCST1-AS1 调节 miR-29b 甲基化的作用。通过细胞增殖实验分析细胞增殖情况。

结果

研究发现，GBM 中 DCST1-AS1 的上调预示着不良的生存。miR-29b 在 GBM 中下调，与 DCST1-AS1 的表达呈负相关。在 GBM 细胞中，过表达 DCST1-AS1 导致 miR-29b 下调和 miR-29b 基因的甲基化水平增加。过表达 DCST1-AS1 导致细胞增殖增加。此外，过表达 DCST1-AS1 显著逆转了 miR-29b 对癌细胞增殖的抑制作用。

结论

在 GBM 中，DCST1-AS1 可能通过甲基化下调 miR-29b 以促进癌细胞增殖。

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