• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

长链非编码RNA SLC16A1-AS1在胶质母细胞瘤中上调，并通过调节miR-149甲基化促进癌细胞增殖。

LncRNA SLC16A1-AS1 is Upregulated in Glioblastoma and Promotes Cancer Cell Proliferation by Regulating miR-149 Methylation.

作者信息

Long Yinbo, Li Heyang, Jin Zhibin, Zhang Xiang

机构信息

Department of Neurosurgery, Cangzhou Central Hospital, Cangzhou City, Hebei Province, 061000, People's Republic of China.

出版信息

Cancer Manag Res. 2021 Feb 10;13:1215-1223. doi: 10.2147/CMAR.S264613. eCollection 2021.

DOI:10.2147/CMAR.S264613
PMID:33603467
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7882451/
Abstract

INTRODUCTION

LncRNA SLC16A1-AS1 has been characterized as a critical player in lung cancer, while its role in glioblastoma (GBM) is unknown. By analyzing the TCGA dataset, we observed the upregulation of SLC16A1-AS1 expression in GBM. Therefore, we aimed to investigate the role of SLC16A1-AS1 in this cancer.

METHODS

GBM tissues and paired non-tumor tissues were collected from 62 GBM patients through biopsy. RT-qPCR was performed to determine the expression of SLC16A1-AS1 and miR-149. Linear regression was used to analyze their correlations. The relationship between SLC16A1-AS1 and miR-149 was assessed by gain and loss of function experiments. Methylation-specific PCR (MSP) and bisulfite sequencing PCR (BSP) were performed to analyze the methylation status of miR-149. Cell proliferation was evaluated by CCK-8 assay and colony formation experiments in GBM cells.

RESULTS

We found that SLC16A1-AS1 expression was upregulated in GBM tissues, and the upregulated expression of SLC16A1-AS1 predicted poor survival of GBM patients. MiR-149 was downregulated in GBM tissues and inversely correlated with the expression of SLC16A1-AS1. In GBM cells, overexpression of SLC16A1-AS1 downregulated the expression of miR-149 and increased the methylation of miR-149 gene. In cell proliferation and colony formation assay, overexpression of SLC16A1-AS1 reduced the inhibitory effects of miR-149 on GBM cell proliferation.

CONCLUSION

SLC16A1-AS1 may promote GBM cell proliferation by regulating miR-149 methylation. SLC16A1-AS1 can be considered as a potential diagnostic marker in GBM.

摘要

引言

长链非编码RNA SLC16A1-AS1已被证实是肺癌中的关键因子，但其在胶质母细胞瘤（GBM）中的作用尚不清楚。通过分析癌症基因组图谱（TCGA）数据集，我们观察到GBM中SLC16A1-AS1表达上调。因此，我们旨在研究SLC16A1-AS1在这种癌症中的作用。

方法

通过活检从62例GBM患者中收集GBM组织和配对的非肿瘤组织。采用逆转录定量聚合酶链反应（RT-qPCR）检测SLC16A1-AS1和miR-149的表达。用线性回归分析它们的相关性。通过功能获得和缺失实验评估SLC16A1-AS1与miR-149之间的关系。采用甲基化特异性聚合酶链反应（MSP）和亚硫酸氢盐测序聚合酶链反应（BSP）分析miR-149的甲基化状态。通过CCK-8法和集落形成实验评估GBM细胞的增殖情况。

结果

我们发现GBM组织中SLC16A1-AS1表达上调，且SLC16A1-AS1表达上调预示着GBM患者预后不良。GBM组织中miR-149表达下调，且与SLC16A1-AS1的表达呈负相关。在GBM细胞中，SLC16A1-AS1的过表达下调了miR-149的表达，并增加了miR-149基因的甲基化。在细胞增殖和集落形成实验中，SLC16A1-AS1的过表达减弱了miR-149对GBM细胞增殖的抑制作用。

结论

SLC16A1-AS1可能通过调节miR-149甲基化促进GBM细胞增殖。SLC16A1-AS1可被视为GBM的潜在诊断标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/308d/7882451/bb75390e5948/CMAR-13-1215-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/308d/7882451/57be259e5ed5/CMAR-13-1215-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/308d/7882451/19809c0d83b8/CMAR-13-1215-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/308d/7882451/bad95491e439/CMAR-13-1215-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/308d/7882451/7628e37986b7/CMAR-13-1215-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/308d/7882451/bb75390e5948/CMAR-13-1215-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/308d/7882451/57be259e5ed5/CMAR-13-1215-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/308d/7882451/19809c0d83b8/CMAR-13-1215-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/308d/7882451/bad95491e439/CMAR-13-1215-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/308d/7882451/7628e37986b7/CMAR-13-1215-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/308d/7882451/bb75390e5948/CMAR-13-1215-g0005.jpg

相似文献

1
LncRNA SLC16A1-AS1 is Upregulated in Glioblastoma and Promotes Cancer Cell Proliferation by Regulating miR-149 Methylation.长链非编码RNA SLC16A1-AS1在胶质母细胞瘤中上调，并通过调节miR-149甲基化促进癌细胞增殖。
Cancer Manag Res. 2021 Feb 10;13:1215-1223. doi: 10.2147/CMAR.S264613. eCollection 2021.
2
LncRNA SLC16A1-AS1 is upregulated in hepatocellular carcinoma and predicts poor survival.长链非编码 RNA SLC16A1-AS1 在肝癌中上调，预测预后不良。
Clin Res Hepatol Gastroenterol. 2021 Mar;45(2):101490. doi: 10.1016/j.clinre.2020.07.001. Epub 2021 Mar 18.
3
MicroRNA-1269 is downregulated in glioblastoma and its maturation is regulated by long non-coding RNA SLC16A1 Antisense RNA 1.微小 RNA-1269 在神经胶质瘤中下调，其成熟受长非编码 RNA SLC16A1 反义 RNA 1 调控。
Bioengineered. 2022 May;13(5):12749-12759. doi: 10.1080/21655979.2022.2070581.
4
LncRNA DCST1-AS1 downregulates miR-29b through methylation in glioblastoma (GBM) to promote cancer cell proliferation.长链非编码 RNA DCST1-AS1 通过甲基化下调胶质母细胞瘤（GBM）中的 miR-29b 促进癌细胞增殖。
Clin Transl Oncol. 2020 Dec;22(12):2230-2235. doi: 10.1007/s12094-020-02363-1. Epub 2020 May 16.
5
LncRNA MAFG-AS1 Suppresses the Maturation of miR-34a to Promote Glioblastoma Cell Proliferation.长链非编码RNA MAFG-AS1抑制miR-34a成熟以促进胶质母细胞瘤细胞增殖。
Cancer Manag Res. 2021 Apr 22;13:3493-3501. doi: 10.2147/CMAR.S274615. eCollection 2021.
6
MiR-526b targets lncRNA SLC16A1-AS1 to suppress cell proliferation in triple-negative breast cancer.miR-526b 通过靶向 lncRNA SLC16A1-AS1 抑制三阴性乳腺癌细胞增殖。
J Biochem Mol Toxicol. 2023 Mar;37(3):e23247. doi: 10.1002/jbt.23247. Epub 2023 Jan 18.
7
LncRNA SLC16A1-AS1 Suppresses Cell Proliferation in Cervical Squamous Cell Carcinoma (CSCC) Through the miR-194/SOCS2 Axis.长链非编码RNA SLC16A1-AS1通过miR-194/SOCS2轴抑制宫颈鳞状细胞癌（CSCC）的细胞增殖。
Cancer Manag Res. 2021 Feb 11;13:1299-1306. doi: 10.2147/CMAR.S276629. eCollection 2021.
8
LncRNA SLC16A1-AS1 regulates the miR-182/PDCD4 axis and inhibits the triple-negative breast cancer cell cycle.长链非编码 RNA SLC16A1-AS1 通过调控 miR-182/PDCD4 轴抑制三阴性乳腺癌细胞周期。
Immunopharmacol Immunotoxicol. 2022 Aug;44(4):534-540. doi: 10.1080/08923973.2022.2056482. Epub 2022 May 2.
9
LncRNA FEZF1-AS1 Sponges miR-34a to Upregulate Notch-1 in Glioblastoma.长链非编码RNA FEZF1-AS1通过海绵吸附miR-34a上调胶质母细胞瘤中的Notch-1
Cancer Manag Res. 2020 Mar 11;12:1827-1833. doi: 10.2147/CMAR.S240531. eCollection 2020.
10
Overexpression of lncRNA SLC16A1-AS1 Suppresses the Growth and Metastasis of Breast Cancer the miR-552-5p/WIF1 Signaling Pathway.长链非编码RNA SLC16A1-AS1的过表达通过miR-552-5p/WIF1信号通路抑制乳腺癌的生长和转移。
Front Oncol. 2022 Mar 15;12:712475. doi: 10.3389/fonc.2022.712475. eCollection 2022.

引用本文的文献

1
The Pivotal Function of SLC16A1 and SLC16A1-AS1 in Cancer Progress: Molecular Pathogenesis and Prognosis.SLC16A1 和 SLC16A1-AS1 在癌症进展中的关键作用：分子发病机制和预后。
Mini Rev Med Chem. 2024;24(18):1685-1700. doi: 10.2174/0113895575284780240327103039.
2
Biological roles of SLC16A1-AS1 lncRNA and its clinical impacts in tumors.SLC16A1-AS1长链非编码RNA的生物学作用及其在肿瘤中的临床影响
Cancer Cell Int. 2024 Mar 30;24(1):122. doi: 10.1186/s12935-024-03285-6.
3
EP300 regulates the SLC16A1-AS1-AS1/TCF3 axis to promote lung cancer malignancies through the Wnt signaling pathway.

本文引用的文献

1
miR-149 Suppresses Breast Cancer Metastasis by Blocking Paracrine Interactions with Macrophages.miR-149 通过阻断与巨噬细胞的旁分泌相互作用抑制乳腺癌转移。
Cancer Res. 2020 Mar 15;80(6):1330-1341. doi: 10.1158/0008-5472.CAN-19-1934. Epub 2020 Jan 7.
2
Clinical prognostic value of isocitrate dehydrogenase mutation, O-6-methylguanine-DNA methyltransferase promoter methylation, and 1p19q co-deletion in glioma patients.异柠檬酸脱氢酶突变、O-6-甲基鸟嘌呤-DNA甲基转移酶启动子甲基化及1p19q共缺失在胶质瘤患者中的临床预后价值
Ann Transl Med. 2019 Oct;7(20):541. doi: 10.21037/atm.2019.09.126.
3
Novel Function of lncRNA ADAMTS9-AS2 in Promoting Temozolomide Resistance in Glioblastoma via Upregulating the FUS/MDM2 Ubiquitination Axis.
EP300通过Wnt信号通路调节SLC16A1-AS1-AS1/TCF3轴，以促进肺癌恶性肿瘤的发展。
Heliyon. 2024 Mar 7;10(6):e27727. doi: 10.1016/j.heliyon.2024.e27727. eCollection 2024 Mar 30.
4
Shedding light on function of long non-coding RNAs (lncRNAs) in glioblastoma.揭示长链非编码RNA（lncRNAs）在胶质母细胞瘤中的功能。
Noncoding RNA Res. 2024 Feb 6;9(2):508-522. doi: 10.1016/j.ncrna.2024.02.002. eCollection 2024 Jun.
5
lncRNA FAM230B is highly expressed in colorectal cancer and suppresses the maturation of miR-1182 to increase cell proliferation.长链非编码RNA FAM230B在结直肠癌中高表达，并抑制miR-1182的成熟以增加细胞增殖。
Open Med (Wars). 2022 Oct 3;17(1):1559-1567. doi: 10.1515/med-2022-0500. eCollection 2022.
6
A novel LUAD prognosis prediction model based on immune checkpoint-related lncRNAs.一种基于免疫检查点相关长链非编码RNA的新型肺腺癌预后预测模型。
Front Genet. 2022 Sep 21;13:1016449. doi: 10.3389/fgene.2022.1016449. eCollection 2022.
7
MicroRNA-1269 is downregulated in glioblastoma and its maturation is regulated by long non-coding RNA SLC16A1 Antisense RNA 1.微小 RNA-1269 在神经胶质瘤中下调，其成熟受长非编码 RNA SLC16A1 反义 RNA 1 调控。
Bioengineered. 2022 May;13(5):12749-12759. doi: 10.1080/21655979.2022.2070581.
8
A long noncoding RNA GTF2IRD2P1 suppresses cell proliferation in bladder cancer by inhibiting the Wnt/β‑catenin signaling pathway.长非编码 RNA GTF2IRD2P1 通过抑制 Wnt/β-连环蛋白信号通路抑制膀胱癌中的细胞增殖。
PeerJ. 2022 Apr 11;10:e13220. doi: 10.7717/peerj.13220. eCollection 2022.
9
Monocarboxylate Transporters Are Involved in Extracellular Matrix Remodelling in Pancreatic Ductal Adenocarcinoma.单羧酸转运体参与胰腺导管腺癌的细胞外基质重塑
Cancers (Basel). 2022 Mar 2;14(5):1298. doi: 10.3390/cancers14051298.
10
Silencing lncRNA SLC16A1-AS1 Induced Ferroptosis in Renal Cell Carcinoma Through miR-143-3p/SLC7A11 Signaling.沉默长链非编码 RNA SLC16A1-AS1 通过 miR-143-3p/SLC7A11 信号通路诱导肾细胞癌发生铁死亡。
Technol Cancer Res Treat. 2022 Jan-Dec;21:15330338221077803. doi: 10.1177/15330338221077803.
lncRNA ADAMTS9-AS2通过上调FUS/MDM2泛素化轴促进胶质母细胞瘤对替莫唑胺耐药的新功能
Front Cell Dev Biol. 2019 Oct 2;7:217. doi: 10.3389/fcell.2019.00217. eCollection 2019.
4
Expression and clinical significance of CPS1 in glioblastoma multiforme.脑胶质母细胞瘤中 CPS1 的表达及临床意义。
Curr Res Transl Med. 2019 Nov;67(4):123-128. doi: 10.1016/j.retram.2019.08.003. Epub 2019 Sep 3.
5
Nicotinamide mononucleotide (NMN) supplementation promotes anti-aging miRNA expression profile in the aorta of aged mice, predicting epigenetic rejuvenation and anti-atherogenic effects.烟酰胺单核苷酸（NMN）补充剂可促进老年小鼠主动脉中抗衰老 miRNA 表达谱，预示着表观遗传年轻化和抗动脉粥样硬化作用。
Geroscience. 2019 Aug;41(4):419-439. doi: 10.1007/s11357-019-00095-x. Epub 2019 Aug 28.
6
lncRNA SLC16A1-AS1 as a novel prognostic biomarker in non-small cell lung cancer.长链非编码 RNA SLC16A1-AS1 作为非小细胞肺癌的一种新型预后生物标志物。
J Investig Med. 2020 Jan;68(1):52-59. doi: 10.1136/jim-2019-001080. Epub 2019 Jul 31.
7
Genetic knockout of myosin light chain kinase (MLCK210) prevents cerebral microhemorrhages and attenuates neuroinflammation in a mouse model of vascular cognitive impairment and dementia.肌球蛋白轻链激酶（MLCK210）基因敲除可预防血管性认知障碍和痴呆小鼠模型的脑微出血，并减轻神经炎症。
Geroscience. 2019 Oct;41(5):671-679. doi: 10.1007/s11357-019-00072-4. Epub 2019 May 19.
8
FGFR2-mediated phosphorylation of PTEN at tyrosine 240 contributes to the radioresistance of glioma.FGFR2介导的PTEN第240位酪氨酸磷酸化促进了胶质瘤的放射抗性。
J Cell Commun Signal. 2019 Sep;13(3):279-280. doi: 10.1007/s12079-019-00518-6. Epub 2019 Apr 25.
9
The effects and the mechanisms of autophagy on the cancer-associated fibroblasts in cancer.自噬对癌症相关成纤维细胞在癌症中的作用和机制。
J Exp Clin Cancer Res. 2019 Apr 23;38(1):171. doi: 10.1186/s13046-019-1172-5.
10
SIRT5 downregulation is associated with poor prognosis in glioblastoma.SIRT5 下调与胶质母细胞瘤的不良预后相关。
Cancer Biomark. 2019;24(4):449-459. doi: 10.3233/CBM-182197.