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细胞表面波形蛋白在Neuro-2a 细胞中 Chandipura 病毒复制中的作用。

Role of cell surface vimentin in Chandipura virus replication in Neuro-2a cells.

机构信息

National Institute of Virology, Kerala Unit, TDMC Hospital complex, Vandanam, Alappuzha, Kerala, 688005, India.

Deartment of Biochemistry, Shivaji University, Vidyanagari, Kolhapur, Maharashtra, 416004, India.

出版信息

Virus Res. 2020 Aug;285:198014. doi: 10.1016/j.virusres.2020.198014. Epub 2020 May 8.

DOI:10.1016/j.virusres.2020.198014
PMID:32418904
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7270567/
Abstract

The neurotropic behavior of Chandipura virus (CHPV) is partly understood in experimental animals. Under in vitro conditions, neuronal cells could be a useful tool to study the CHPV interaction with neuronal proteins. The information gathered from such studies will help to design the new therapeutics for CHPV infection. This study identified the surface vimentin protein involved in adsorption of CHPV on Neuro-2a cell line (mouse neuroblastoma cells). The decrease in CHPV infectivity to Neuro-2a cells was observed in the presence of recombinant vimentin or anti-vimentin antibody. Vimentin mRNA expression remains unaltered in CHPV infected Neuro-2a cells. Furthermore, in silico analysis predicted the residues in vimentin and CHPV glycoprotein (G); probably involved in cell-virus interactions. Overall, we conclude that surface vimentin in Neuro-2a cells interact with CHPV and facilitate the binding of CHPV to the cells; it could be acting as a co-receptor for the CHPV. Further investigation is necessary to confirm the exact role of vimentin in CHPV infection in neuronal cells.

摘要

钱德里帕拉病毒（CHPV）的神经趋向性行为在实验动物中得到了部分理解。在体外条件下，神经元细胞可能是研究 CHPV 与神经元蛋白相互作用的有用工具。从这些研究中收集的信息将有助于设计针对 CHPV 感染的新疗法。本研究鉴定了参与 CHPV 在Neuro-2a 细胞系（小鼠神经母细胞瘤细胞）上吸附的表面波形蛋白。在存在重组波形蛋白或抗波形蛋白抗体的情况下，观察到 CHPV 对 Neuro-2a 细胞的感染性降低。CHPV 感染的 Neuro-2a 细胞中的波形蛋白 mRNA 表达保持不变。此外，计算机分析预测了波形蛋白和 CHPV 糖蛋白（G）中的残基；可能参与细胞-病毒相互作用。总的来说，我们得出结论，Neuro-2a 细胞表面的波形蛋白与 CHPV 相互作用，并促进 CHPV 与细胞的结合；它可能作为 CHPV 的共受体。需要进一步的研究来证实波形蛋白在神经元细胞中 CHPV 感染的确切作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38c1/7270567/7e9a43edcf69/gr7_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38c1/7270567/774a31654975/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38c1/7270567/df818bef97b4/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38c1/7270567/fece05b2273b/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38c1/7270567/741ef4473441/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38c1/7270567/d07cb6a75c9c/gr5_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38c1/7270567/17c5ad6204df/gr6_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38c1/7270567/7e9a43edcf69/gr7_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38c1/7270567/774a31654975/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38c1/7270567/df818bef97b4/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38c1/7270567/fece05b2273b/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38c1/7270567/741ef4473441/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38c1/7270567/d07cb6a75c9c/gr5_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38c1/7270567/17c5ad6204df/gr6_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38c1/7270567/7e9a43edcf69/gr7_lrg.jpg

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