Hriouech Soukayna, Akhmouch Ahmed A, Mzabi Aouatef, Chefchaou Hanane, Tanghort Mariam, Oumokhtar Bouchra, Chami Najat, Remmal Adnane
Laboratory of Biotechnology, Faculty of Sciences Dhar El Mehraz, University Sidi Mohamed Ben Abdallah, BP 1796 Atlas, 30 050 Fez, Morocco.
Laboratory of Microbiology and Molecular Biology, Faculty of Medicine and Pharmacy, University Sidi Mohammed Ben Abdallah, BP 1893 Route Sidi Harazem, 30 070 Fez, Morocco.
Evid Based Complement Alternat Med. 2020 Apr 28;2020:4271017. doi: 10.1155/2020/4271017. eCollection 2020.
The aim of this research paper is to test the antistaphylococcal effect of 1,8-cineole, amoxicillin/clavulanic acid (AMC), and gentamicin, either separately or in combination against three strains isolated from patients suffering from osteomyelitis. This activity was tested by using the microdilution method and the checkerboard assay. The efficacy of these three antibacterial agents was then tested by using an experimental model of methicillin-resistant osteomyelitis in rabbits. This efficacy was assessed after four days of treatment by counting the number of bacteria in the bone marrow. The obtained results showed that the combination of the AMC with gentamicin did not induce a synergistic effect, whereas the combination of the two antibiotics with 1,8-cineole did. This effect is stronger when AMC is combined with 1,8-cineole as a total synergistic effect was obtained on the three strains used (FIC ≤ 0.5). , a significant reduction was noted in the number of colonies in the bone marrow when rabbits were treated with AMC associated with either 1,8-cineole or gentamicin compared to rabbits treated with AMC, gentamicin, or 1,8-cineole alone. These results demonstrated that 1,8-cineole showed a synergistic effect in combination with both AMC and gentamicin, which offer possibilities for reducing antibiotic usage. Also, the AMC associated with 1,8-cineole could be used to treat MRSA osteomyelitis.
本研究论文的目的是测试1,8-桉叶素、阿莫西林/克拉维酸（AMC）和庆大霉素单独或联合使用对从骨髓炎患者分离出的三株菌株的抗葡萄球菌效果。通过微量稀释法和棋盘法检测该活性。然后使用兔耐甲氧西林骨髓炎实验模型测试这三种抗菌剂的疗效。治疗四天后，通过计算骨髓中的细菌数量评估疗效。所得结果表明，AMC与庆大霉素的组合未产生协同效应，而两种抗生素与1,8-桉叶素的组合产生了协同效应。当AMC与1,8-桉叶素联合使用时，这种效应更强，因为在所使用的三株菌株上均获得了完全协同效应（FIC≤0.5）。与单独用AMC、庆大霉素或1,8-桉叶素治疗的兔子相比，用AMC与1,8-桉叶素或庆大霉素联合治疗的兔子骨髓中的菌落数量显著减少。这些结果表明，1,8-桉叶素与AMC和庆大霉素联合使用时均显示出协同效应，这为减少抗生素使用提供了可能性。此外，AMC与1,8-桉叶素联合可用于治疗耐甲氧西林金黄色葡萄球菌骨髓炎。
