Sienkiewicz Monika, Łysakowska Monika, Kowalczyk Edward, Szymańska Grażyna, Kochan Ewa, Krukowska Jolanta, Olszewski Jurek, Zielińska-Bliźniewska Hanna
Department of Allergology and Respiratory Rehabilitation, 2nd Chair of Otolaryngology, Medical University of Lodz, Poland.
Department of Microbiology and Medical Laboratory Immunology, Medical University of Lodz, Poland.
Burns. 2017 Mar;43(2):310-317. doi: 10.1016/j.burns.2016.08.032. Epub 2016 Oct 27.
The aim of this work was to characterize the ability of essential oils to support antibiotics against pathogenic bacteria in wounds. Gram-positive and Gram-negative bacteria obtained from wound infections were identified according to standard microbiological methods. Essential oils were analysed by GC-FID-MS. The susceptibility of bacteria to antibiotics, essential oils and their combination was assessed using the disc-diffusion method. The Minimal Inhibitory Concentration and Minimum Bactericidal Concentration of the essential oils were established by the micro-dilution broth method. Although cinnamon, clove, thyme and lavender essential oils were found to have the greatest antibacterial activity when used alone, the greatest additive and synergistic effects against pathogenic wound bacteria in combination with recommended antibiotics were demonstrated by basil, clary sage and rosemary oils.
这项工作的目的是表征精油在伤口中协同抗生素对抗病原菌的能力。根据标准微生物学方法鉴定从伤口感染中分离出的革兰氏阳性菌和革兰氏阴性菌。采用气相色谱-氢火焰离子化检测器-质谱联用仪（GC-FID-MS）分析精油。使用纸片扩散法评估细菌对抗生素、精油及其组合的敏感性。通过微量稀释肉汤法确定精油的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。虽然单独使用时发现肉桂、丁香、百里香和薰衣草精油具有最强的抗菌活性，但罗勒、鼠尾草和迷迭香精油与推荐抗生素联合使用时，对伤口病原菌表现出最大的相加和协同作用。
