使用突变菌株在搅拌罐反应器中生产木聚糖酶的工艺参数和发酵策略的优化

Optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutant strain.

作者信息

Abdella Asmaa, Segato Fernando, Wilkins Mark R

机构信息

Department of Industrial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, PO Box 79, Sadat City, 22857 Egypt.

Department of Biological Systems Engineering, University of Nebraska-Lincoln, 3605 Fair Street, Lincoln, NE, 68583-0726 USA.

出版信息

Biotechnol Rep (Amst). 2020 Apr 22;26:e00457. doi: 10.1016/j.btre.2020.e00457. eCollection 2020 Jun.

The present work studied the optimization of aeration rate, agitation rate and oxygen transfer and the use of various batch fermentation strategies for xylanase production from a recombinant strain in a 3 L stirred tank reactor. Maximum xylanase production of 1250 U/mL with productivity of 313 U/mL/day was obtained under an aeration rate of 2 vvm and an agitation rate of 400 rpm using batch fermentation. The optimum volumetric oxygen transfer coefficient (ka) for efficient xylanase production was found to be 38.6 h. Fed batch mode and repeated batch fermentation was also performed with ka was 38.6 h. Xylanase enzyme productivity increased to 327 with fed batch fermentation and 373 U/mL/day with repeated batch fermentation. Also, maximum xylanase activity increased to 1410 U/mL with fed batch fermentation and 1572 U/mL with repeated batch fermentation.

本研究工作在3升搅拌罐式反应器中，对通气速率、搅拌速率和氧传递进行了优化，并研究了使用各种分批发酵策略从重组菌株生产木聚糖酶的情况。在分批发酵中，通气速率为2 vvm、搅拌速率为400 rpm时，木聚糖酶的最大产量为1250 U/mL，生产率为313 U/mL/天。发现有效生产木聚糖酶的最佳体积氧传递系数（ka）为38.6 h⁻¹。在ka为38.6 h⁻¹的情况下，还进行了补料分批发酵模式和重复分批发酵。补料分批发酵时木聚糖酶的酶生产率提高到327 U/mL/天，重复分批发酵时提高到373 U/mL/天。此外，补料分批发酵时木聚糖酶的最大活性提高到1410 U/mL，重复分批发酵时提高到1572 U/mL。