Iturralde Esteban Tomás, Stocco Marina Celeste, Faura Andrés, Mónaco Cecilia Inés, Cordo Cristina, Pérez-Giménez Julieta, Lodeiro Aníbal Roberto
Laboratorio de Interacciones entre Rizobios y Soja (Lirys), IBBM CCT-La Plata CONICET and Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calles 47 y 115 (1900), La Plata, Argentina.
CIDEFI, CIC-PBA and Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, Calles 60 y 119 (1900), La Plata, Argentina.
Biotechnol Rep (Amst). 2020 Apr 29;26:e00461. doi: 10.1016/j.btre.2020.e00461. eCollection 2020 Jun.
Coinoculation of plants with mixtures of beneficial microbes sometimes produces synergistic effects. In this study, the effect of soybean coinoculation with the N-fixing E109 and the biocontrol fungus Th5cc was analyzed. Nodulation by E109 was not hampered by Th5cc, which antagonized five out of seven soybean pathogens tested. Furthermore, Th5cc relieved nitrate-inhibition of nodulation, enabling the formation of nodules containing infected cells with bacteroids in the presence of the otherwise inhibitory 10 mM KNO. Th5cc released micromolar amounts of auxin, and addition of 11 μM indoleacetic acid to soybean plants inoculated with E109 in the absence of Th5cc also induced nodulation in the presence of 10 mM KNO. Thus, Th5cc may release auxins into the soybean rhizosphere, which hormones might participate in overcoming the nitrate-inhibition of nodulation. Our results suggest that soybean plants coinoculated with these microorganisms might benefit from biocontrol while contributing to soil-nitrogen preservation.
将有益微生物混合物与植物共同接种有时会产生协同效应。在本研究中，分析了大豆与固氮菌E109和生防真菌Th5cc共同接种的效果。E109的结瘤未受到Th5cc的阻碍，Th5cc对所测试的七种大豆病原体中的五种具有拮抗作用。此外，Th5cc缓解了硝酸盐对结瘤的抑制作用，使得在存在原本具有抑制作用的10 mM KNO₃时能够形成含有被类菌体感染细胞的根瘤。Th5cc释放出微摩尔量的生长素，并且在没有Th5cc的情况下向接种E109的大豆植株添加11 μM吲哚乙酸也能在10 mM KNO₃存在时诱导结瘤。因此，Th5cc可能会向大豆根际释放生长素，这些激素可能参与克服硝酸盐对结瘤的抑制作用。我们的结果表明，与这些微生物共同接种的大豆植株可能会从生物防治中受益，同时有助于土壤氮素的保存。
