Departamento de Biotecnología, Universidad Autónoma Metropolitana, Unidad Iztapalapa, CP 09340, Iztapalapa, México, D.F., Mexico.
Equipe_Eco technologies et Bioremédiation, Aix Marseille Université, IMBE-UMR CNRS-7263/IRD-237, Case 421, Campus Etoile, Faculté St JérÔme, 13397 Marseille Cedex 20, France.
Bioresour Technol. 2018 Oct;265:291-298. doi: 10.1016/j.biortech.2018.06.015. Epub 2018 Jun 14.
A novel bench-scale stirred bioreactor for solid-state fermentation was used to determine the effect of the stirring rate on growth and enzymes production by Trichoderma harzianum PBLA. Lab-scale static tubular bioreactors were first used to assess the effect of bioreactor diameter on heat accumulation, growth, and production of cellulases and xylanases. The increased diameters (1.8-4.2 cm) led to increases in temperature up to 36 °C (at a rate of 1.08 °C/cm), which negatively affected the growth and enzyme production. Afterward, in the bench-scale bioreactor operated at rates up to 3.0 rpm, maximum xylanases production (107 ± 0.3 U/g dm) was attained at rates of 0.5 and 1.0 rpm, reaching a maximum of 34 ± 0.3 °C. Cellulases production was reduced (up to 79%) due to stirring. Therefore, the production of xylanases by T. harzianum can be performed in this cross-flow stirred SSF bioreactor at rates up to 1.0 rpm, avoiding heat accumulation and damage on metabolic activity.
一种新型的用于固态发酵的搅拌式生物反应器被用于确定搅拌速率对哈茨木霉 PBLA 生长和酶生产的影响。实验室规模的静态管状生物反应器首先用于评估生物反应器直径对热量积累、生长以及纤维素酶和木聚糖酶生产的影响。直径的增加（1.8-4.2cm）导致温度上升高达 36°C（速率为 1.08°C/cm），这对生长和酶生产产生负面影响。此后，在以高达 3.0rpm 的速率运行的台式生物反应器中，在 0.5 和 1.0rpm 的速率下，木聚糖酶的最大产量（107±0.3U/gdm）达到 34±0.3°C。由于搅拌，纤维素酶的产量降低（高达 79%）。因此，哈茨木霉的木聚糖酶生产可以在这种横流搅拌固态发酵生物反应器中以高达 1.0rpm 的速率进行，避免热量积累和对代谢活性的损害。
