el Fari M, Gräser Y, Presber W, Tietz H J
Department of Microbiology and Hygiene, Humboldt University, Berlin, Germany.
Mycoses. 2000;43(5):191-6. doi: 10.1046/j.1439-0507.2000.00558.x.
An outbreak of tinea corporis due to Trichophyton tonsurans among 46 children (aged 7-17 years) was investigated. Most of them were wrestlers. Thirty-one strains were identified by conventional methods, but proved to be problematic in 15 isolates due to colony variation and reduced sporulation. They were identified as Trichophyton tonsurans by the use of molecular methods, for example, sequence comparison of the ribosomal internal transcribed spacer (ITS) region and polymerase chain reaction fingerprinting. No DNA polymorphisms were detected with any of the techniques used, suggesting clonal reproduction of the populations of the species and providing evidence for spatial and temporal stability of the lineage.
对46名儿童(7至17岁)中由断发毛癣菌引起的体癣暴发进行了调查。他们大多数是摔跤运动员。通过传统方法鉴定出31株菌株,但由于菌落变异和孢子形成减少,15株分离株鉴定存在问题。通过分子方法,例如核糖体内部转录间隔区(ITS)区域的序列比较和聚合酶链反应指纹图谱,将它们鉴定为断发毛癣菌。所使用的任何技术均未检测到DNA多态性,这表明该物种群体的克隆繁殖,并为该谱系的时空稳定性提供了证据。