基于 UPLC-MS/MS 代谢谱分析优化从 L. 中提取血管紧张素转化酶抑制剂的提取溶剂。

Optimization of an Extraction Solvent for Angiotensin-Converting Enzyme Inhibitors from L. Based on Its UPLC-MS/MS Metabolic Profiling.

机构信息

Department of Pharmacognosy, Faculty of Pharmacy, Menoufia University, Gamal Abd El Nasr st., Shibin Elkom 32511, Egypt.

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Ain-Shams University, Cairo 11566, Egypt.

出版信息

Molecules. 2020 May 14;25(10):2307. doi: 10.3390/molecules25102307.

species (Malvaceae) have been long used as an antihypertensive folk remedy. The aim of our study was to specify the optimum solvent for extraction of the angiotensin-converting enzyme inhibiting (ACEI) constituents from L. The 80% methanol extract (H2) showed the highest ACEI activity, which exceeds that of the standard captopril (IC50 0.01255 ± 0.00343 and 0.210 ± 0.005 µg/mL, respectively). Additionally, in a comprehensive metabolomics approach, an ultra-performance liquid chromatography (UPLC) coupled to the high resolution tandem mass spectrometry (HRMS) method was used to trace the metabolites from each extraction method. Interestingly, our comprehensive analysis showed that the 80% methanol extract was predominated with secondary metabolites from all classes including flavonoids, anthocyanins, phenolic and organic acids. Among the detected metabolites, phenolic acids such as ferulic and chlorogenic acids, organic acids such as citrate derivatives and flavonoids such as kaempferol have been positively correlated to the antihypertensive potential. These results indicates that these compounds may significantly contribute synergistically to the ACE inhibitory activity of the 80% methanol extract.

锦葵科（Malvaceae）的植物一直被用作民间的降血压草药。我们的研究目的是确定提取具有血管紧张素转化酶抑制（ACEI）作用的成分的最佳溶剂。80%甲醇提取物（H2）显示出最高的 ACEI 活性，超过了标准的卡托普利（IC50 分别为 0.01255 ± 0.00343 和 0.210 ± 0.005 µg/mL）。此外，在全面的代谢组学方法中，使用超高效液相色谱（UPLC）结合高分辨串联质谱（HRMS）方法来追踪每种提取方法的代谢物。有趣的是，我们的综合分析表明，80%甲醇提取物主要含有来自所有类别的次生代谢物，包括类黄酮、花青素、酚类和有机酸。在所检测到的代谢物中，类黄酮如槲皮素和绿原酸，有机酸如柠檬酸衍生物，以及类黄酮如山柰酚，都与降血压的潜力呈正相关。这些结果表明，这些化合物可能对 80%甲醇提取物的 ACE 抑制活性有协同增效作用。