Pharmacognosy Department, Faculty of Pharmacy, October 6 University, Egypt.
Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, Weinberg 3, D 06120 Halle Saale, Germany.
Food Chem. 2018 Jun 1;250:236-244. doi: 10.1016/j.foodchem.2018.01.020. Epub 2018 Jan 3.
Roselle (Hibiscus sabdariffa) is a functional food with potential health benefits, consumed either as hot or cold beverage. To ensure quality control of its various products, accurate measurement of active metabolites is warranted. Herein, we propose a combination of ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) and nuclear magnetic resonance (NMR) analytical platforms for the untargeted characterization of metabolites in two roselle cultivars, Aswan and Sudan-1. The analyses revealed 33 metabolites, including sugars, flavonoids, anthocyanins, phenolic and aliphatic organic acids. Their relative contents in cultivars were assessed via principle component analysis (PCA) and orthogonal projection to latent structures analysis (OPLS). Impact of the different extraction methods (decoction, infusion and maceration) was compared by quantitative H NMR spectroscopy, revealing cold maceration to be optimal for preserving anthocyanins, whereas infusion was more suited for recovering organic acids. The metabolite pattern revealed by the different extraction methods was found in good correlation for their ability to inhibit α-glucosidase enzyme.
洛神花（ Hibiscus sabdariffa ）是一种具有潜在健康益处的功能性食品，可作为热饮或冷饮食用。为了确保其各种产品的质量控制，有必要对活性代谢物进行准确测量。在此，我们提出了一种超高效液相色谱-质谱联用（UHPLC-MS）和核磁共振（NMR）分析平台的组合，用于对两个洛神花品种（Aswan 和 Sudan-1）的代谢物进行非靶向特征分析。分析共鉴定出 33 种代谢物，包括糖、类黄酮、花青素、酚类和脂类有机酸。通过主成分分析（PCA）和正交偏最小二乘判别分析（OPLS）评估了品种中各代谢物的相对含量。通过定量 1 H NMR 光谱比较了不同提取方法（煎煮、浸泡和浸渍）的影响，结果表明冷浸法更有利于保持花青素，而浸泡法更适合回收有机酸。不同提取方法的代谢物图谱与其抑制α-葡萄糖苷酶的能力呈良好相关性。
