Sanada S, Arai E, Ueda M, Ogura K, Yoshida O
Department of Urology, Kansai-denryoku Hospital.
Hinyokika Kiyo. 1988 Nov;34(11):1965-72.
A number of studies have implied that seminal plasma contains one or more factors that stimulate spermatozoal motility. On the other hand, many authors have shown that continuous, long term exposure to seminal plasma is detrimental to motility and survival of spermatozoa. In this study we examined the long term effects of human seminal plasma not only from whole semen but also from different fractions of split ejaculate on motility of human ejaculated spermatozoa. Washed spermatozoa were incubated in modified BWW medium containing human serum albumin with or without seminal plasma. A considerable percentage of spermatozoa maintained good progressive motility for up to 20 h. when free from seminal plasma. Addition of seminal plasma (20%, v/v) obviously depressed the motility, particularly progressive motility. The most depressive effect occurred in the first (prostatic) fraction of split ejaculate, and the effect of the last (vesicular) fraction was less harmful. These observations suggest that seminal plasma has (a) factor(s) that impair the maintenance of spermatozoal motility which originate possibly from the prostate or epididymis, since both fluids of these accessory organs appear to be secreted mainly into the first portion ejaculate.
多项研究表明，精浆中含有一种或多种刺激精子活力的因子。另一方面，许多作者已经证明，持续、长期接触精浆对精子的活力和存活是有害的。在本研究中，我们不仅研究了全精液中的人类精浆，还研究了分段射精不同部分的精浆对人类射出精子活力的长期影响。将洗涤后的精子在含有或不含有精浆的添加人血清白蛋白的改良BWW培养基中孵育。相当比例的精子在没有精浆的情况下，可保持良好的前向运动能力长达20小时。添加精浆（20%，v/v）明显降低了精子活力，尤其是前向运动能力。最具抑制作用的是分段射精的第一部分（前列腺部分），而最后一部分（精囊部分）的影响较小。这些观察结果表明，精浆中存在一种或多种损害精子活力维持的因子，这些因子可能起源于前列腺或附睾，因为这些附属器官的液体似乎主要分泌到射精的第一部分。
