Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.
Department of Integrated Structural Biosciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan.
Mol Reprod Dev. 2020 Jun;87(6):663-665. doi: 10.1002/mrd.23349. Epub 2020 May 19.
Using auto-erasable Sendai virus vector, we generated ciPSC line. After several passages, virus was not present in ciPSCs by RT-PCR. ciPSCs from canine PBMCs had pluripotent state, differentiated all three germ layers in vitro, and had normal 78 XX karyotype. These results proved that PBMCs were one of the good cell sources to generate ciPSC lines from companion and patient dogs.
利用可自动清除的仙台病毒载体，我们生成了 ciPSC 系。通过 RT-PCR 检测，经过几轮传代后，ciPSC 中已不存在病毒。来自犬 PBMC 的 ciPSC 具有多能性，能够在体外分化为三个胚层，并且具有正常的 78 XX 核型。这些结果证明 PBMC 是从伴侣动物和患病犬中生成 ciPSC 系的良好细胞来源之一。
