Kong Wenqian, Nabukalu Pheonah, Cox T Stan, Goff Valorie H, Pierce Gary J, Lemke Cornelia, Robertson Jon S, Compton Rosana, Tang Haibao, Paterson Andrew H
Plant Genome Mapping Laboratory, University of Georgia, Athens, GA, United States.
The Land Institute, Salina, KS, United States.
Front Plant Sci. 2020 Apr 30;11:467. doi: 10.3389/fpls.2020.00467. eCollection 2020.
Despite a "ploidy barrier," interspecific crosses to wild and/or cultivated sorghum (, 2n = 2x = 20) may have aided the spread across six continents of , also exemplifying risks of "transgene escape" from crops that could make weeds more difficult to control. Genetic maps of two BCF populations derived from crosses of (sorghum) and with totals of 722 and 795 single nucleotide polymorphism (SNP) markers span 37 and 35 linkage groups, with 2-6 for each of the 10 basic sorghum chromosomes due to fragments covering different chromosomal portions or independent segregation from different homologs. Segregation distortion favored alleles on chromosomes 2 (1.06-4.68 Mb, near a fertility restoration gene), 7 (1.20-6.16 Mb), 8 (1.81-5.33 Mb, associated with gene conversion), and 9 (47.5-50.1 Mb); and alleles on chromosome 6 (0-40 Mb), which contains both a large heterochromatin block and the gene. Regions of the genome that are recalcitrant to gene flow from sorghum might be exploited as part a multi-component system to reduce the likelihood of spread of transgenes or other modified genes. Its SNP profile suggests that chromosome segments from its respective progenitors and have extensively recombined in . This study reveals genomic regions that might discourage crop-to-weed gene escape, and provides a foundation for marker-trait association analysis to determine the genetic control of traits contributing to weediness, invasiveness, and perenniality of .
尽管存在“倍性障碍”，但与野生和/或栽培高粱（2n = 2x = 20）的种间杂交可能有助于[物种名称]在六大洲的传播，这也例证了作物“转基因逃逸”的风险，这种逃逸可能会使杂草更难控制。由[物种名称1]（高粱）与[物种名称2]杂交产生的两个回交导入系群体的遗传图谱，分别有722个和795个单核苷酸多态性（SNP）标记，跨越37个和35个连锁群，由于片段覆盖不同的染色体部分或来自不同[物种名称2]同源染色体的独立分离，10条基本高粱染色体中的每条染色体有2 - 6个连锁群。分离畸变有利于2号染色体（1.06 - 4.68兆碱基，靠近育性恢复基因）、7号染色体（1.20 - 6.16兆碱基）、8号染色体（1.81 - 5.33兆碱基，与基因转换相关）和9号染色体（47.5 - 50.1兆碱基）上的[物种名称1]等位基因；以及6号染色体（0 - 40兆碱基）上的[物种名称2]等位基因，该染色体包含一个大的异染色质块和[基因名称]基因。[物种名称1]基因组中难以从高粱进行基因流动的区域，可作为多组分系统的一部分加以利用，以降低转基因或其他修饰基因扩散的可能性。其SNP图谱表明，来自其各自亲本[物种名称1]和[物种名称2]的染色体片段在[物种名称1]中广泛重组。本研究揭示了可能抑制作物向杂草基因逃逸的基因组区域，并为标记 - 性状关联分析奠定了基础，以确定影响[物种名称1]杂草性、入侵性和多年生性的性状的遗传控制。
