Li Yuanfei, Li Yuqi, Jin Wei, Sharpton Thomas J, Mackie Roderick I, Cann Isaac, Cheng Yanfen, Zhu Weiyun
Laboratory of Gastrointestinal Microbiology, National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, China.
Joint International Research Laboratory of Animal Health and Food Safety, Nanjing Agricultural University, Nanjing, China.
Front Microbiol. 2019 Mar 6;10:435. doi: 10.3389/fmicb.2019.00435. eCollection 2019.
In this study, the effects of a syntrophic methanogen on the growth of sp. F1 was investigated by characterizing fermentation profiles, as well as functional genomic, transcriptomic, and proteomic analysis. The estimated genome size, GC content, and protein coding regions of strain F1 are 106.83 Mb, 16.07%, and 23.54%, respectively. Comparison of the fungal monoculture with the methanogen co-culture demonstrated that during the fermentation of glucose, the co-culture initially expressed and then down-regulated a large number of genes encoding both enzymes involved in intermediate metabolism and plant cell wall degradation. However, the number of up-regulated proteins doubled at the late-growth stage in the co-culture. In addition, we provide a mechanistic understanding of the metabolism of this fungus in co-culture with a syntrophic methanogen. Further experiments are needed to explore this interaction during degradation of more complex plant cell wall substrates.
在本研究中，通过表征发酵谱以及进行功能基因组学、转录组学和蛋白质组学分析，研究了一种互营产甲烷菌对菌株F1生长的影响。菌株F1的估计基因组大小、GC含量和蛋白质编码区分别为106.83 Mb、16.07%和23.54%。真菌单培养与产甲烷菌共培养的比较表明，在葡萄糖发酵过程中，共培养最初表达然后下调了大量编码参与中间代谢和植物细胞壁降解的酶的基因。然而，在共培养的生长后期，上调蛋白质的数量增加了一倍。此外，我们提供了对这种真菌与互营产甲烷菌共培养时代谢机制的理解。需要进一步的实验来探索在更复杂的植物细胞壁底物降解过程中的这种相互作用。
