分枝杆菌中霉菌酸裂解产物的气相色谱分析
Gas-chromatographic analysis of mycolic acid cleavage products in mycobacteria.
作者信息
Guerrant G O, Lambert M A, Moss C W
出版信息
J Clin Microbiol. 1981 May;13(5):899-907. doi: 10.1128/jcm.13.5.899-907.1981.
Abstract
Mycolic acids were detected in both reference strains and clinical isolates of mycobacteria using gas chromatography of fatty acid methyl esters prepared by acid methanolysis. The methyl esters were extracted with hexane, concentrated, and analyzed with a gas chromatograph by using two different injector temperatures. When the samples were analyzed at high injector temperatures of 300 to 350 degrees C, characteristic thermal cleavage products from mycolic acids, C22:0, C24:0, or C26:0 fatty acid methyl esters, were detected. When analyzed at injector temperatures of 235 degrees C or lower, the mycolic acids were heat stable and the characteristic methyl ester cleavage products were not observed.
摘要
通过对酸甲醇解制备的脂肪酸甲酯进行气相色谱分析，在分枝杆菌的参考菌株和临床分离株中均检测到了分枝菌酸。甲酯用己烷萃取、浓缩，然后使用两种不同的进样器温度通过气相色谱仪进行分析。当样品在300至350摄氏度的高进样器温度下分析时，检测到了分枝菌酸、C22:0、C24:0或C26:0脂肪酸甲酯的特征性热裂解产物。当在235摄氏度或更低的进样器温度下分析时，分枝菌酸具有热稳定性，未观察到特征性甲酯裂解产物。
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