Department of Chemistry, University of Rome Tor Vergata, Via della Ricerca Scientifica, 00133, Rome, Italy.
Chemistry. 2020 Aug 6;26(44):9826-9834. doi: 10.1002/chem.202001660. Epub 2020 Jun 29.
The fundamental concept of effective molarity is observed in a variety of biological processes, such as protein compartmentalization within organelles, membrane localization and signaling paths. To control molecular encountering and promote effective interactions, nature places biomolecules in specific sites inside the cell in order to generate a high, localized concentration different from the bulk concentration. Inspired by this mechanism, scientists have artificially recreated in the lab the same strategy to actuate and control artificial DNA-based functional systems. Here, it is discussed how harnessing effective molarity has led to the development of a number of proximity-induced strategies, with applications ranging from DNA-templated organic chemistry and catalysis, to biosensing and protein-supported DNA assembly.
有效摩尔浓度的基本概念在多种生物过程中都有体现，如细胞器内的蛋白质区室化、膜定位和信号通路。为了控制分子的相遇并促进有效相互作用，自然界将生物分子安置在细胞内的特定位置，以产生与体相浓度不同的高局部浓度。受此机制的启发，科学家们在实验室中人工重现了相同的策略，以激活和控制基于人工 DNA 的功能系统。在这里，讨论了如何利用有效摩尔浓度来开发许多接近诱导策略，其应用范围从 DNA 模板有机化学和催化，到生物传感和蛋白质支持的 DNA 组装。
