Department of Biology, Katholieke Universiteit Leuven, 3000 Leuven, Belgium.
Mayurbhanj Biological Research (MBR), Bhanjpur, Baripada 757002, Odisha, India.
Viruses. 2020 May 15;12(5):549. doi: 10.3390/v12050549.
Chikungunya and yellow fever virus cause vector-borne viral diseases in humans. There is currently no specific antiviral drug for either of these diseases. Banana plants are used in traditional medicine for treating viral diseases such as measles and chickenpox. Therefore, we tested selected banana cultivars for their antiviral but also cytotoxic properties. Different parts such as leaf, pseudostem and corm, collected separately and extracted with four different solvents (hexane, acetone, ethanol, and water), were tested for in vitro antiviral activity against Chikungunya virus (CHIKV), enterovirus 71 (EV71), and yellow fever virus (YFV). Extracts prepared with acetone and ethanol from leaf parts of several cultivars exhibited strong (EC around 10 μg/mL) anti-CHIKV activity. Interestingly, none of the banana plant extracts (concentration 1-100 µg/mL) were active against EV71. Activity against YFV was restricted to two cultivars: Namwa Khom-Pseudostem-Ethanol (5.9 ± 5.4), Namwa Khom-Corm-Ethanol (0.79 ± 0.1) and Fougamou-Corm-Acetone (2.5 ± 1.5). In most cases, the cytotoxic activity of the extracts was generally 5- to 10-fold lower than the antiviral activity, suggesting a reasonable therapeutic window.
基孔肯雅热和黄热病病毒可引起人类虫媒病毒性疾病。目前，针对这两种疾病尚无特效抗病毒药物。香蕉植物在传统医学中用于治疗麻疹和水痘等病毒性疾病。因此，我们测试了一些特定的香蕉品种，以评估它们的抗病毒和细胞毒性特性。我们分别采集了香蕉植物的叶、假茎和球茎等不同部位，用四种不同溶剂（正己烷、丙酮、乙醇和水）提取，然后在体外检测它们对基孔肯雅热病毒（CHIKV）、肠道病毒 71（EV71）和黄热病病毒（YFV）的抗病毒活性。结果表明，几种品种的叶部用丙酮和乙醇提取的提取物对 CHIKV 具有很强的活性（EC 值约为 10μg/mL）。有趣的是，香蕉植物的提取物（浓度为 1-100μg/mL）对 EV71 均无活性。对 YFV 的活性仅局限于两个品种：Namwa Khom-假茎-乙醇（5.9 ± 5.4），Namwa Khom-球茎-乙醇（0.79 ± 0.1）和 Fougamou-球茎-丙酮（2.5 ± 1.5）。在大多数情况下，提取物的细胞毒性活性通常比抗病毒活性低 5-10 倍，表明有合理的治疗窗口。
