Master in Biomedical Sciences, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Indonesia.
Department of Physiology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Indonesia.
Iran Biomed J. 2020 Sep;24(5):281-7. doi: 10.29252/ibj.24.5.276. Epub 2020 Jan 20.
Inflammatory bowel disease is a chronic inflammatory condition associated with damage to the intestinal mucosal barrier. Supplementation of yacon tubers has been known to have positive effect on intestinal health. Therefore, we conducted the present study to investigate the effect of yacon tuber powder on Th1 activation pathway by evaluating IFN-γ levels and the number of goblet cells in the colon of colitis mouse models.
Thirty BALB/c mice were divided into five groups: 1, control group (G1) and 2-5, TNBS-induced colitis groups (GII-GV). Yacon powder was given to three of the TNBS-induced colitis groups with the doses of 0.165 g/30 g BW (GIII), 0.331 g/30 g body weight (BW; GIV), and 0.662 g/30 g BW (GV) for 14 days. IFN-γ levels were assessed using ELISA, while number of goblet cells was calculated based on histological observation.
Significantly lower IFN-γ levels was observed in GV compared to colitis group (GII) (p = 0.007). GV also showed significantly higher number of goblet cells per 100 epithelial cells in 20 crypts (p = 0.000) than GII.
The administration of yacon powder at a dose of 0.662 g/30 g BW could decrease IFN-γ levels and improve the healing of intestinal mucosa in colitis mouse models by increasing the number of goblet cells.
炎症性肠病是一种与肠黏膜屏障损伤相关的慢性炎症性疾病。已知食用雪莲果对肠道健康有积极影响。因此，我们进行了本研究，通过评估 IFN-γ 水平和结肠杯状细胞数量来研究雪莲果粉对结肠炎小鼠模型 Th1 激活途径的影响。
将 30 只 BALB/c 小鼠分为 5 组：1、对照组（G1）和 2-5、TNBS 诱导的结肠炎组（GII-GV）。将雪莲果粉给予 3 个 TNBS 诱导的结肠炎组，剂量分别为 0.165 g/30 g BW（GIII）、0.331 g/30 g 体重（BW；GIV）和 0.662 g/30 g BW（GV），共 14 天。通过 ELISA 评估 IFN-γ 水平，通过组织学观察计算杯状细胞数量。
GV 组 IFN-γ 水平明显低于结肠炎组（GII）（p=0.007）。GV 组 20 个隐窝中每 100 个上皮细胞的杯状细胞数量也明显高于 GII 组（p=0.000）。
在结肠炎小鼠模型中，每天给予 0.662 g/30 g BW 的雪莲果粉可降低 IFN-γ 水平，并通过增加杯状细胞数量改善肠道黏膜的愈合。
