Bastaki Salim M A, Al Ahmed Mohammed Majed, Al Zaabi Ahmed, Amir Naheed, Adeghate Ernest
Department of Pharmacology, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain, UAE.
Department of Anatomy, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain, UAE.
BMC Complement Altern Med. 2016 Feb 23;16:72. doi: 10.1186/s12906-016-1057-5.
This study investigates the protective effects of turmeric (Curcuma longa, CL) on acetic acid-induced colitis in rats.
Inflammatory bowel disease (IBD) was induced in male Wistar rats by intra-rectal administration of 1 ml of 4% acetic acid at 8 cm proximal to the anus for 30 s. Curcuma longa (CL) powder, (1, 10, or 100 mg/kg/day) was administered for either 3 days before or after IBD for 7 days. The body weight, macroscopic and microscopic analysis of the colon of CL-treated IBD rats and that of control rats (no IBD, no CL) were performed on 0 day, 2, 4 and 7th day. Myeloperoxidase (MPO), IL-23 and glutathione levels in control, untreated and treated rats were measured by ELISA.
CL significantly (P < 0.05) improved IBD-induced reduction in mean body weight and mean macroscopic ulcer score. Administration of CL also significantly (P < 0.01) reduced the mean microscopic ulcer score when compared to untreated IBD control. Intake of CL by rats resulted in a significant (P < 0.05) increase in the mean serum glutathione level compared to untreated control. CL reduced both MPO and IL-23 levels in the colonic mucosa of the rat.
CL improved body weight gain, mean macroscopic and microscopic ulcer scores in the colon of rats suffering from acetic acid-induced IBD. CL reduced both MPO and IL-23 in the mucosa of the colon. The increase in the mean serum glutathione level may help in the reduction of oxidative stress associated with IBD.
本研究调查姜黄（Curcuma longa，CL）对大鼠乙酸诱导性结肠炎的保护作用。
通过在距肛门8厘米处直肠内注射1毫升4%乙酸30秒，诱导雄性Wistar大鼠患炎症性肠病（IBD）。在IBD诱导前3天或诱导后7天，给予姜黄（CL）粉末（1、10或100毫克/千克/天）。在第0天、第2天、第4天和第7天，对接受CL治疗的IBD大鼠和对照大鼠（无IBD，无CL）的体重、结肠进行宏观和微观分析。通过酶联免疫吸附测定法（ELISA）测量对照、未治疗和治疗大鼠中的髓过氧化物酶（MPO）、白细胞介素-23（IL-23）和谷胱甘肽水平。
CL显著（P < 0.05）改善了IBD诱导的平均体重减轻和平均宏观溃疡评分。与未治疗的IBD对照相比，给予CL也显著（P < 0.01）降低了平均微观溃疡评分。与未治疗的对照相比，大鼠摄入CL导致平均血清谷胱甘肽水平显著（P < 0.05）升高。CL降低了大鼠结肠黏膜中的MPO和IL-23水平。
CL改善了乙酸诱导性IBD大鼠的体重增加、结肠的平均宏观和微观溃疡评分。CL降低了结肠黏膜中的MPO和IL-23。平均血清谷胱甘肽水平的升高可能有助于降低与IBD相关的氧化应激。
