Dong Tingjun, Lu Zejun, Li Jingjiao, Liu Yongzhen, Wen Juyi
The Second School of Clinical Medicine, Southern Medical University, Guangzhou 510280, China.
Tumor Diagnosis and Treatment Center, The Sixth Medical Center of PLA General Hospital, Beijing 100048, China.
Zhongguo Fei Ai Za Zhi. 2020 May 20;23(5):306-313. doi: 10.3779/j.issn.1009-3419.2020.104.17.
Flubendazole is an anthelmintic and categorized in benzimidazole. Previous evidence indicates its suppression on proliferation of colon cancer and breast cancer cells. Our study aims to explore the effects of flubendazole on non-small cell lung cancer A549 and H460 cell lines and the underlying mechanism.
CCK-8 assay was used to detect the effect of flubendazole at different concentrations on viability of both cell lines A549 and H460. We used western blot to detect the expression levels of autophagy-related proteins p62 and LC3 after flubendazole treatment. Cells were transfected with tandem fluorescent adenovirus (mRFP-GFP-LC3), and the impact of flubendazole treatment on autophagic flux were analyzed.
Cell viability analysis showed a dose-dependent inhibitory effect on proliferation of both A549 and H460, comparing to cells without flubendazole treating (P<0.001). Level of p62 decreased and LC3 II/I ratio increased in cells treated with 2 μmol/L flubendazole for 24 h and 48 h, compared to control groups (P<0.005). Red fluorescence signals increased in mRFP-GFP-LC3 transfected cells after flubendazole treating, suggesting an elevation in autophagic flux.
Flubendazole may inhibit the proliferation of A549 and H460 cells and promote autophagy.
氟苯达唑是一种抗蠕虫药，属于苯并咪唑类。先前的证据表明其对结肠癌细胞和乳腺癌细胞的增殖有抑制作用。我们的研究旨在探讨氟苯达唑对非小细胞肺癌A549和H460细胞系的影响及其潜在机制。
采用CCK-8法检测不同浓度氟苯达唑对A549和H460两种细胞系活力的影响。我们使用蛋白质免疫印迹法检测氟苯达唑处理后自噬相关蛋白p62和LC3的表达水平。用串联荧光腺病毒（mRFP-GFP-LC3）转染细胞，并分析氟苯达唑处理对自噬流的影响。
细胞活力分析显示，与未用氟苯达唑处理的细胞相比，氟苯达唑对A549和H460细胞的增殖均有剂量依赖性抑制作用（P<0.001）。与对照组相比，用2μmol/L氟苯达唑处理24小时和48小时的细胞中，p62水平降低，LC3 II/I比值升高（P<0.005）。氟苯达唑处理后，mRFP-GFP-LC3转染细胞中的红色荧光信号增加，表明自噬流升高。
氟苯达唑可能抑制A549和H460细胞的增殖并促进自噬。
