Department of Electronic Science, University of Calcutta, 92 A.P.C. Road, Kolkata, 700009, India.
Department of Physiology, University of Calcutta, 92 A.P.C. Road, Kolkata, 700009, India.
Biomed Microdevices. 2020 May 20;22(2):38. doi: 10.1007/s10544-020-00493-5.
An on-chip device has been fabricated on Si platform for precise estimation of the hematocrit (Hct) level of human blood with rapid turn out. Impedance/capacitance spectroscopy and current-voltage (I-V) measurements have been employed to observe the variation of electrical parameters of erythrocyte suspensions with varying Hct level. Experimentally obtained values of capacitance, impedance and conductance with Hct level suggests a linear variation. Current-time measurement has also been performed to ensure the susceptibility of red blood cells under a fixed external electric field for certain duration of time. The online real time sample analyzes have also been performed by the device connected with an embedded electronic circuit interfaced with a laptop through appropriate software. This has enabled the development of a novel Si-chip based compact point-of-care (POC) diagnosis system for Hct level estimation by measuring the dielectric/capacitive variation of an erythrocyte cell suspension. The relevant performance parameters of such a compact system including range, resolution, limit of detection and throughput have also been evaluated.
已经在 Si 平台上制造了一种片上设备，用于快速准确估计人体血液的血细胞比容 (Hct) 水平。采用阻抗/电容谱和电流-电压 (I-V) 测量来观察红细胞悬浮液的电参数随 Hct 水平变化的变化。实验获得的电容、阻抗和电导值与 Hct 水平呈线性变化。还进行了电流-时间测量，以确保在固定的外部电场下红细胞在一定时间内的易感性。通过与通过适当软件与笔记本电脑接口的嵌入式电子电路连接的设备，还可以在线实时分析样品。这使得通过测量红细胞悬浮液的介电/电容变化来开发一种基于 Si 芯片的新型紧凑型即时护理 (POC) 诊断系统成为可能，用于估计 Hct 水平。还评估了这种紧凑型系统的相关性能参数，包括范围、分辨率、检测限和吞吐量。
