Key Laboratory of Organic Optoelectronics & Molecular Engineering of the Ministry of Education, Department of Chemistry, Tsinghua University, Beijing, 100084, China.
Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
Angew Chem Int Ed Engl. 2020 Aug 24;59(35):15176-15180. doi: 10.1002/anie.202005334. Epub 2020 Jun 9.
The shape of eukaryotic cells is determined by the cytoskeleton associated with membrane proteins; however, the detailed mechanism of how the integral morphologies with structural stability is generated and maintained is still not fully understood. Here, based on the Frame-Guided Assembly (FGA) strategy, we successfully prepared hetero-liposomes with structural composition similar to that of eukaryotic cells by screening a series of transmembrane peptides as the leading hydrophobic groups (LHGs). It was demonstrated that the conformation and transmembrane mode of the LHGs played dominant roles during the FGA process. The FGA liposomes were formed with excellent stability, which may further provide evidence for the cytoskeleton-membrane protein-lipid bilayer model. Taking advantage of the biocompatibility and stability, the FGA liposomes were also applied to prepare novel drug delivery vehicles, which is promising in diagnostic imaging and cancer therapy applications.
真核细胞的形状由与膜蛋白相关的细胞骨架决定；然而，对于具有结构稳定性的整体形态如何产生和维持的详细机制，仍不完全清楚。在这里，我们基于框架引导组装（FGA）策略，通过筛选一系列作为主导疏水区（LHGs）的跨膜肽，成功制备了具有类似于真核细胞结构组成的异质脂质体。结果表明，LHGs 的构象和跨膜模式在 FGA 过程中起主导作用。FGA 脂质体具有优异的稳定性，这可能进一步为细胞骨架-膜蛋白-脂质双层模型提供证据。利用其生物相容性和稳定性，FGA 脂质体还被应用于制备新型药物递送载体，在诊断成像和癌症治疗应用中具有广阔的前景。
