Key Laboratory of Organic Optoelectronics & Molecular Engineering of the Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084 (P. R. China); Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Molecular Recognition and Function, Institute of Chemistry, Chinese Academy of Sciences (CAS), Beijing 100190 (P. R. China).
Angew Chem Int Ed Engl. 2014 Dec 1;53(49):13468-70. doi: 10.1002/anie.201408231. Epub 2014 Oct 5.
We report a thermally triggered frame-guided assembly (FGA) strategy for the preparation of vesicles. We employ thermally responsive poly(propylene oxide) (PPO) to make the leading hydrophobic groups (LHGs) thermally responsive, so that they are hydrophilic below the low critical solution temperature (LCST) and the frame forms in a homogeneous environment. When the temperature is increased above the LCST, the LHGs become hydrophobic and the assembly process is triggered, which drives DNA-b-PPO to assemble around the LHGs, forming vesicles. This work verified that FGA is a general strategy and can be applied to polymeric systems. The thermally triggered assembly not only provides more controllability over the FGA process but also promotes an in-depth understanding of the FGA strategy and in a broad view, the formation mechanism and functions of cell membrane.
我们报告了一种热触发框架引导组装（FGA）策略，用于制备囊泡。我们利用热响应性聚（环氧丙烷）（PPO）使主导疏水区（LHGs）热响应，使其在低临界溶液温度（LCST）以下亲水，并且在均相环境中形成框架。当温度升高到 LCST 以上时，LHGs 变得疏水，组装过程被触发，这促使 DNA-b-PPO 围绕 LHGs 组装，形成囊泡。这项工作验证了 FGA 是一种通用策略，可应用于聚合物体系。热触发组装不仅为 FGA 过程提供了更多的可控性，而且促进了对 FGA 策略以及更广泛的细胞膜形成机制和功能的深入理解。
