Fernandes Fabiano A N, Fonteles Thatyane V, Rodrigues Sueli, de Brito Edy Sousa, Tiwari Brijesh K
1Departamento de Engenharia Química, Universidade Federal do Ceara, Campus do Pici, Bloco 709, Fortaleza, CE 60440-900 Brazil.
2Departamento de Engenharia de Alimentos, Universidade Federal do Ceara, Campus do Pici, Bloco 858, Fortaleza, CE 60440-900 Brazil.
J Food Sci Technol. 2020 Jun;57(6):2321-2328. doi: 10.1007/s13197-020-04270-3. Epub 2020 Jan 29.
Jabuticaba () is a dark berry, endemic to the south and central regions of South America, rich in anthocyanins and polyphenols. This study evaluated the ultrasound-assisted extraction of bioactive compounds from jabuticaba peel, developed a new mathematical model for the process, and estimated the model parameters. Extraction was carried out using water as solvent aiming its direct use in food formulations. The main anthocyanin (cyanidin-3--glucoside) and the main polyphenol (ellagic acid) from jabuticaba peel were extracted and quantified by LC-MS and HPLC. The results indicate that lowering the pH increased the extraction of the anthocyanin and had only slight effect on the extraction of ellagic acid. The application of ultrasound at 25 kHz favored the extraction of both compounds. Processing time of 20 min increased the yield of both compounds, while over processing (> 20 min) let to the sonochemical-induced hydrolysis of cyanidin-3--glucoside and ellagic acid. The highest yield of bioactive compounds was attained at 25 kHz, 20 min of extraction and pH 1.5 (8.9 mg/g dry peel of gallic acid equivalent, 0.9 mg/g dry peel of ellagic acid, and 7.9 mg/g dry peel of cyanidin-3--glucoside). The new mathematical model considered the mass transfer between the powder and the liquid media, and the sonochemical-induced hydrolysis of the compound. The model was able to predict satisfactorily the extraction process and the hydrolysis effect.
嘉宝果（）是一种深色浆果，原产于南美洲的南部和中部地区，富含花青素和多酚。本研究评估了超声辅助从嘉宝果果皮中提取生物活性化合物的方法，开发了该过程的新数学模型，并估算了模型参数。提取过程以水为溶剂，旨在直接用于食品配方。通过液相色谱 - 质谱联用仪（LC - MS）和高效液相色谱法（HPLC）对嘉宝果果皮中的主要花青素（矢车菊素 - 3 - 葡萄糖苷）和主要多酚（鞣花酸）进行提取和定量。结果表明，降低pH值可提高花青素的提取率，而对鞣花酸的提取仅有轻微影响。25kHz的超声处理有利于两种化合物的提取。20分钟的处理时间提高了两种化合物的产率，而过度处理（>20分钟）会导致矢车菊素 - 3 - 葡萄糖苷和鞣花酸发生声化学诱导水解。在25kHz、提取20分钟和pH值为1.5的条件下，生物活性化合物的产率最高（没食子酸当量为8.9mg/g干果皮、鞣花酸为0.9mg/g干果皮、矢车菊素 - 3 - 葡萄糖苷为7.9mg/g干果皮）。新的数学模型考虑了粉末与液体介质之间的传质以及化合物的声化学诱导水解。该模型能够令人满意地预测提取过程和水解效果。
