UNICAMP - University of Campinas, School of Food Engineering, LANUM, Rua Monteiro Lobato, 80, Campinas, SP CEP:13083-862, Brazil.
INRAE, Avignon University, UMR408 SQPOV, F-84000 Avignon, France.
Food Chem. 2021 Oct 15;359:129959. doi: 10.1016/j.foodchem.2021.129959. Epub 2021 Apr 27.
Ultrasound-assisted and solvent extractions resulted in similar levels of hydrolyzable tannins (10.3-6.0 mg/g), anthocyanins (7.8-10.2 mg/g) and flavonols (0.24-0.32 mg/g) for dried Myrciaria jaboticaba peel (DJP). Ultrasound was efficient for the extraction of poorly soluble hydrolyzable tannins but affected the stability of anthocyanins and flavonols. UPLC-DAD-MS allowed the identification of 44 hydrolyzable tannins as single and mixed hexosides bearing galloyl, HHDP and tergalloyl units. Twelve mixed HHDP-galloylgluconic acids and tergalloylated hexosides were newly discovered in this work. Acid hydrolysis of both ultrasonic extract and DJP yielded five major compounds, i.e. gallic acid, ellagic acid, gallic acid-C-hexoside, valoneic acid dilactone and sanguisorbic acid dilactone and pointed to higher contents in hydrolyzable tannins than by summing individual polyphenols after UPLC. Last, cyanidin-3-O-glucoside and hydrolyzable tannins from the ultrasonic extract inhibited lipid peroxidation of a Western type meal in in vitro digestion, suggesting a health benefit for these jabuticaba polyphenols.
超声辅助提取和溶剂提取对干燥的穗状醋栗果皮（DJP）中可水解单宁（10.3-6.0mg/g）、花色苷（7.8-10.2mg/g）和类黄酮（0.24-0.32mg/g）的提取效果相当。超声提取对提取难溶性可水解单宁很有效，但会影响花色苷和类黄酮的稳定性。UPLC-DAD-MS 鉴定出 44 种可水解单宁，为带有没食子酰基、HHDP 和倍儿茶酰基单元的单糖和混合己糖苷。本研究新发现了 12 种混合 HHDP-没食子酰基葡萄糖酸和倍儿茶酰化己糖苷。超声提取和 DJP 的酸水解均得到了 5 种主要化合物，即没食子酸、鞣花酸、没食子酰基葡萄糖、缬草酸二内酯和血根堿二内酯，这表明可水解单宁中的含量高于 UPLC 后单独计算多酚的总和。最后，超声提取物中的矢车菊素-3-O-葡萄糖苷和可水解单宁抑制了西式餐在体外消化中的脂质过氧化，表明这些穗状醋栗多酚具有健康益处。
