Ribarac-Stepić N, Kovacina K, Zamaklar M, Devecerski M
Institute for Nuclear Sciences Boris Kidric, Faculty of Medicine, Belgrade, Yugoslavia.
Endocrinol Exp. 1988 Dec;22(4):203-9.
The erythrocytes were used as a model system to study insulin receptors in diabetic patients with highly increased insulin resistance. The specific binding of 125I-insulin to erythrocytes of these patients was markedly reduced. When erythrocytes prepared from non-diabetic subjects were exposed to serum of these patients, the decrease of insulin binding was reproduced. The binding of insulin showed a displacement curve that is parallel to the control values over the same range of hormone concentration, even in the case when control erythrocytes were preincubated with serum of diabetics with increased insulin resistance. These results indicate the presence of certain component in blood plasma of examined patients which alters insulin binding to receptors. The developed assay provides an efficient method for detection and identification of substances presented in the serum which can influence the binding of insulin to the specific sites as well as its biological effects.
红细胞被用作一个模型系统，以研究胰岛素抵抗大幅增加的糖尿病患者的胰岛素受体。这些患者红细胞对125I胰岛素的特异性结合明显减少。当用非糖尿病受试者制备的红细胞暴露于这些患者的血清中时，胰岛素结合的减少得以重现。即使在对照红细胞与胰岛素抵抗增加的糖尿病患者血清预孵育的情况下，胰岛素结合在相同激素浓度范围内也呈现出与对照值平行的置换曲线。这些结果表明，所检测患者的血浆中存在某种成分，它会改变胰岛素与受体的结合。所开发的检测方法为检测和鉴定血清中可能影响胰岛素与特定位点结合及其生物学效应的物质提供了一种有效的方法。
