Korpachev V V, Gurina N M, Ivanova Zh V
Ukr Biokhim Zh (1978). 1994 Jul-Aug;66(4):65-8.
The alteration of indices of 125-I insulin binding to receptors in the reactions with human erythrocytes, with their membranes and with solubilized insulin receptors in the presence of type 2 diabetics' blood serum was demonstrated (decrease of the number of insulin binding sites and increase of the dissociation constant). Treatment of patients' erythrocytes with acetate buffer solution (0.1 M, pH 5, 150 mM sodium chloride) restored the level of specific binding of insulin to normal values. It is supposed that in insulin-independent diabetes mellitus patients' serum there are specific blocking factors which interact immediately with insulin receptors thus preventing them from hormone binding or reducing their affinity.
在2型糖尿病患者血清存在的情况下，证明了125-I胰岛素与人类红细胞、其细胞膜以及可溶性胰岛素受体反应中胰岛素与受体结合指标的改变（胰岛素结合位点数量减少，解离常数增加）。用醋酸盐缓冲溶液（0.1M，pH5，150mM氯化钠）处理患者红细胞可使胰岛素特异性结合水平恢复至正常。推测在非胰岛素依赖型糖尿病患者血清中存在特异性阻断因子，其直接与胰岛素受体相互作用，从而阻止受体与激素结合或降低其亲和力。
