Department of Biology, UNESP (São Paulo State University), IB (Instituto de Biociências), Rio Claro, São Paulo, Brazil.
J Environ Sci Health B. 2020;55(8):687-693. doi: 10.1080/03601234.2020.1766898. Epub 2020 May 20.
The objective of this research was to investigate the potential damage caused by the residual concentrations of the insecticides Regent WS 800 and Curbix SC 200, containing fipronil and ethiprole, respectively as active ingredients, on the liver of . The analyses of HSP70 shock protein labelling and cell death process by TUNEL method were performed in order to measure the effects of the exposure of cell repair system of fish to both insecticides. Statistical analyses showed no significant molecular damage to the hepatic tissue of animals. Nevertheless, variations in HSP70 and DNA fragmentation levels, endpoint of cell repair system response and cellular death, respectively, were observed in several groups. These results indicate that the cell repair machinery was efficient when in contact with residual concentrations of insecticides. However, the DNA fragmentation detected by the TUNEL method suggests that even in face of the cytoprotective action of the HSP70 protein, there are damages that become irreparable. To finish, it is worth mentioning that given the results obtained from residual concentrations, use in the field should be with caution.
本研究的目的是调查残留浓度的杀虫剂 Regent WS 800 和 Curbix SC 200 对. 的肝脏可能造成的损害，这两种杀虫剂分别含有氟虫腈和乙基多杀菌素作为活性成分。为了测量鱼类细胞修复系统暴露于这两种杀虫剂的影响，进行了 HSP70 应激蛋白标记和 TUNEL 法细胞死亡过程的分析。统计分析显示，动物肝组织没有明显的分子损伤。然而，在几个组中观察到 HSP70 和 DNA 片段化水平的变化，分别为细胞修复系统反应和细胞死亡的终点。这些结果表明，当接触到残留浓度的杀虫剂时，细胞修复机制是有效的。然而，TUNEL 法检测到的 DNA 片段化表明，即使有 HSP70 蛋白的细胞保护作用，仍有不可修复的损伤。最后，值得一提的是，考虑到残留浓度的结果，在田间使用时应谨慎。
