Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai 201399, People's Republic of China.
The First Affiliated Hospital of the Harbin Medical University, Heilongjiang Province 150001, People's Republic of China.
IET Nanobiotechnol. 2020 Apr;14(2):120-125. doi: 10.1049/iet-nbt.2018.5342.
An accurate, safe, environmentally friendly, fast and sensitive electrochemical biosensors were developed to detect xanthine in serum. The metal-organic framework ZIF-8 was synthesised and elemental gold was supported on the surface of ZIF-8 by reduction method to synthesise Ag-ZIF-8. The mesoporous carbon material and the synthesised Ag-ZIF-8 were, respectively, applied to a glassy carbon electrode to construct biosensors. The constructed biosensor has a good linear relation in the range of 1-280 μmol l of xanthine and the detection limit is 0.167 μmol l. The relative standard deviation value in serum samples was <5%, and the recoveries were 96-106%, indicating the good selectivity, stability and reproducibility of this electrochemical biosensor.
开发了一种准确、安全、环保、快速且灵敏的电化学生物传感器，用于检测血清中的黄嘌呤。通过还原法合成了金属有机骨架 ZIF-8，并将元素金负载在 ZIF-8 表面上，合成了 Ag-ZIF-8。将介孔碳材料和合成的 Ag-ZIF-8 分别应用于玻碳电极构建生物传感器。构建的生物传感器在黄嘌呤 1-280 μmol/L 的范围内具有良好的线性关系，检测限为 0.167 μmol/L。血清样品中的相对标准偏差值<5%，回收率为 96-106%，表明该电化学生物传感器具有良好的选择性、稳定性和重现性。
