Tumor Immunology Lab, Department of Pathology, Rikshospitalet, Oslo University Hospital and University of Oslo, Oslo, Norway.
Department of Cardiothoracic Surgery, Ullevål Hospital, Oslo University Hospital, Oslo, Norway.
Scand J Immunol. 2020 Aug;92(2):e12893. doi: 10.1111/sji.12893. Epub 2020 Jun 9.
Pulmonary typical carcinoid (TC) is a low-grade, rare lung cancer of neuroendocrine origin. Currently, there is very little information available about the immune cell composition in TC tumours. Here, we analysed by flow cytometry resected tumours from four never-smoker female patients with TC. Twelve distinct immune cell types were identified in TC tumours. The most abundant immune cells were CD8 T cells, CD4 T cells, B cells and macrophages, which represented 19.8%, 17.7%, 11.5% and 11% of all tumour-infiltrating CD45 leucocytes, respectively. Natural killer (NK) cells (8.8%) and neutrophils (3.9%) were also common. Three types of dendritic cells (DCs) were identified (plasmacytoid DCs, CD1c DCs, and CD141 DCs) which together constituted 1.4% of all immune cells in TC tumours. Small populations of basophils (1.2%), mast cells (0.8%) and eosinophils (0.6%) were also present. Notably, the percentage of leucocytes (of all living cells) was much lower in TC tumours compared to high-grade non-small cell lung cancer (NSCLC) tumours and also compared to non-cancerous lung tissue. We conclude that TC tumours are relatively non-inflammatory, although the immune landscape was found to be very complex.
肺典型类癌（TC）是一种起源于神经内分泌的低度恶性罕见肺癌。目前，关于 TC 肿瘤中的免疫细胞组成的信息非常有限。在这里，我们通过流式细胞术分析了 4 名从不吸烟的女性 TC 患者的切除肿瘤。在 TC 肿瘤中鉴定出 12 种不同的免疫细胞类型。最丰富的免疫细胞是 CD8 T 细胞、CD4 T 细胞、B 细胞和巨噬细胞，分别占所有肿瘤浸润性 CD45 白细胞的 19.8%、17.7%、11.5%和 11%。自然杀伤 (NK) 细胞 (8.8%) 和中性粒细胞 (3.9%) 也很常见。鉴定出三种树突状细胞 (DC)（浆细胞样 DC、CD1c DC 和 CD141 DC），它们共同构成 TC 肿瘤中所有免疫细胞的 1.4%。还存在少量的嗜碱性粒细胞 (1.2%)、肥大细胞 (0.8%) 和嗜酸性粒细胞 (0.6%)。值得注意的是，与高级别非小细胞肺癌 (NSCLC) 肿瘤和非癌性肺组织相比，TC 肿瘤中的白细胞（所有活细胞的百分比）要低得多。我们得出结论，TC 肿瘤相对非炎症性，尽管免疫景观被发现非常复杂。
