Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education , Chengdu, China.
Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization , Sichuan Province, Chengdu, China.
Biosci Biotechnol Biochem. 2020 Aug;84(8):1594-1602. doi: 10.1080/09168451.2020.1767497. Epub 2020 May 20.
KLF9 is reported to promote adipocyte differentiation in 3T3-L1 cells and pigs. However, the roles of KLF9 in adipocytes differentiation of goat remain unknown. In this study, the expression profiles of KLF9 were different between subcutaneous and intramuscular preadipocytes of goat during differentiation process. After silencing KLF9 gene, the lipid droplets were increased in both two types of adipocytes. In subcutaneous preadipocyte with silencing KLF9, the expressions of C/EBPβ, PPARγ, LPL, KLF1-2, KLF5, and KLF17 genes were up-regulated, while KLF12, KLF4, and KLF13 genes were down-regulated in expression level. In intramuscular preadipocyte, aP2, C/EBPα, KLF2-3, KLF5, and KLF7 gene were up-regulated, and Pref-1 gene was down-regulated. In addition, the binding sites of KLF9 existed in the promoters of aP2, C/EBPα, C/EBPβ, LPL and Pref-1. Taken together, KLF9 play a negative role in the differentiation of both intramuscular and subcutaneous preadipocytes in goats, but the functional mechanism may be different.
KLF9 被报道可促进 3T3-L1 细胞和猪的脂肪细胞分化。然而，KLF9 在山羊脂肪细胞分化中的作用尚不清楚。本研究在山羊脂肪前体细胞分化过程中，发现 KLF9 在皮下和肌内前体脂肪细胞中的表达谱不同。沉默 KLF9 基因后，两种脂肪细胞中的脂滴都增加了。在沉默 KLF9 的皮下前体脂肪细胞中，C/EBPβ、PPARγ、LPL、KLF1-2、KLF5 和 KLF17 基因的表达上调，而 KLF12、KLF4 和 KLF13 基因的表达下调。在肌内前体脂肪细胞中，aP2、C/EBPα、KLF2-3、KLF5 和 KLF7 基因上调，Pref-1 基因下调。此外，KLF9 的结合位点存在于 aP2、C/EBPα、C/EBPβ、LPL 和 Pref-1 的启动子中。综上所述，KLF9 在山羊肌内和皮下前体脂肪细胞的分化中发挥负调控作用，但功能机制可能不同。
