FGF1 promotes the differentiation of goat intramuscular and subcutaneous preadipocytes.

Fibroblast growth factor 1(1) has been proved to bind to specific signal molecules and activate intracellular signal transduction, leading to proliferation or differentiation of cells. However, the role of 1 in goat adipocytes is still unclear. Here, we investigated its role in lipogenesis of goats, which depends on the activation of FGFRs. In goat intramuscular and subcutaneous adipocytes, we observed that adipocytes accumulation was inhibited by interfering of 1, the expression of /α, /β, , -1, γ, 2, 4, 6, 10 and 17 were significantly down-regulated ( < 0.05). When the 1 was up-regulated, the opposite result was found, while the expression of /β, , γ, 1, 2, 4, 7, 15, 16 and 17 were increased significantly ( < 0.05) in goat intramuscular and subcutaneous adipocytes. The expression level of 1 was significantly and decreased with the interference of 1, and increased with the overexpression of 1. But in goat subcutaneous adipocytes, only the expression of 2 was consistent with the expression of 1. Interference methods confirmed that 1 or 2 and 1 have the similarly promoting function in adipocytes differentiation. With the co-transfection technology, we confirmed that 1 promoted the differentiation of intramuscular and subcutaneous adipocytes might via 1 or 2, respectively.