Cvjetinovic Julijana, Salimon Alexey I, Novoselova Marina V, Sapozhnikov Philipp V, Shirshin Evgeny A, Yashchenok Alexey M, Kalinina Olga Yu, Korsunsky Alexander M, Gorin Dmitry A
Center for Photonics and Quantum Materials, Skolkovo Institute of Science and Technology, 3 Nobelya Str., Moscow, 121205, Russia.
Center for Energy Science and Technology, Skolkovo Institute of Science and Technology, 3 Nobelya Str., Moscow, 121205, Russia.
Photoacoustics. 2020 Mar 10;18:100171. doi: 10.1016/j.pacs.2020.100171. eCollection 2020 Jun.
Photoacoustic and fluorescent methods are used intensely in biology and medicine. These approaches can also be used to investigate unicellular diatom algae that are extremely important for Earth's ecology. They are enveloped within silica frustules (exoskeletons), which can be used in drug delivery systems. Here, we report for the first time the successful application of photoacoustic (PA) and fluorescent visualization of diatoms. Chlorophyll and and fucoxanthin were found likely to be responsible for the photoacoustic effect in diatoms. The PA signal was obtained from gel drops containing diatoms and was found to increase with the diatom concentration. The fluorescence lifetime of the diatom chromophores ranged from 0.5 to 2 ns. The dynamic light scattering, absorbance, and SEM characterization techniques were also applied. The results were considered in combination to elucidate the nature of the photoacoustic signal. Possible biotechnological applications are proposed for the remote photoacoustic monitoring of algae.
光声和荧光方法在生物学和医学中得到了广泛应用。这些方法也可用于研究对地球生态极为重要的单细胞硅藻。它们被包裹在硅质壳（外骨骼）内，硅质壳可用于药物递送系统。在此，我们首次报告了光声（PA）和荧光可视化硅藻的成功应用。发现叶绿素a、叶绿素c和岩藻黄质可能是硅藻光声效应的原因。从含有硅藻的凝胶滴中获得了PA信号，并且发现该信号随硅藻浓度的增加而增强。硅藻发色团的荧光寿命范围为0.5至2纳秒。还应用了动态光散射、吸光度和扫描电子显微镜表征技术。综合考虑这些结果以阐明光声信号的性质。提出了藻类远程光声监测的可能生物技术应用。
