银杏叶提取物对 HCN 基因编码的起搏通道的影响。
Effect of Ginkgo biloba extract on pacemaker channels encoded by HCN gene.
机构信息
Department of Cardiology, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuchang District, 430060, Wuhan, Hubei Province, China.
Cardiovascular Research Institute, Wuhan University, 430060, Wuhan, China.
出版信息
Herz. 2021 Jun;46(3):255-261. doi: 10.1007/s00059-020-04933-z. Epub 2020 May 20.
BACKGROUND
In the present study, the electropharmacological activity of traditional Chinese medicine, Ginkgo biloba extract (GBE), on human hyperpolarization-activated nucleotide-gated (HCN) channels and the underlying "funny" currents was investigated.
METHODS
Standard two-electrode voltage-clamp recordings were employed to examine the properties of cloned HCN subunit currents expressed in Xenopus oocytes under controlled conditions and GBE administration.
RESULTS
We found that GBE irreversibly inhibited the HCN2 and HCN4 channel currents in a concentration-dependent fashion and that the HCN4 current was more sensitive to GBE compared with HCN2. In addition, GBE inhibition of the current amplitudes of HCN2 and HCN4 currents was accompanied by a decrease in the activation and deactivation kinetics.
CONCLUSION
The results of this study contribute toward illustrating the antiarrhythmic mechanism of GBE, which might be useful for the treatment of arrhythmia.
背景
在本研究中，研究了传统中药银杏叶提取物（GBE）对人超极化激活的核苷酸门控（HCN）通道和潜在的“有趣”电流的电药理学活性。
方法
采用标准的双电极电压钳记录技术，在控制条件下和 GBE 给药下，研究在非洲爪蟾卵母细胞中表达的克隆 HCN 亚基电流的特性。
结果
我们发现，GBE 以浓度依赖的方式不可逆地抑制 HCN2 和 HCN4 通道电流，并且与 HCN2 相比，HCN4 电流对 GBE 更敏感。此外，GBE 抑制 HCN2 和 HCN4 电流的幅度伴随着激活和失活动力学的降低。
结论
本研究的结果有助于阐明 GBE 的抗心律失常机制，这可能对心律失常的治疗有用。
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