Zhang Wei, Han Lei, Xing Peng, Liu Bailiang, Sun Zhongqi, Zhou Wenping, Dong Jiahong
Department of Hepatobiliary Surgery, General Hospital of Northern Theater Command, No.83, Wenhua Road, Shenhe District, Shenyang, 110016, China.
Post-doctoral Station, General Hospital of Northern Theater Command, No.83, Wenhua Road, Shenhe District, Shenyang, 110016, China.
Naunyn Schmiedebergs Arch Pharmacol. 2020 Dec;393(12):2543-2551. doi: 10.1007/s00210-020-01889-z. Epub 2020 May 21.
It is reported that long noncoding RNA RHPN1-AS1 (lncRNA RHPN1-AS1) functions as an oncogene among multiple types of cancers; however, the effect of lncRNA RHPN1-AS1 in hepatocellular carcinoma (HCC) is left to be investigated. The main purpose of this work was to study the effects of lncRNA RHPN1-AS1/miR-485-5p system on proliferation, migration, and invasion in HCC and future investigate the latent mechanisms. Our work found that lncRNA RHPN1-AS1 was observably up-regulated in HCC tissues and cell lines, especially HCCLM3 and SMMC-7721 cells. LncRNA RHPN1-AS1 knockdown decreased the capacity of proliferation, invasion, and migration in HCCLM3 and SMMC-7721 cells, which could be crippled by miR-485-5p inhibitor. Besides, the expression of basigin (BSG) was decreased after lncRNA RHPN1-AS1 silence, indicating the function of lncRNA RHPN1-AS1/miR-485-5p/BSG axis in HCC progression. Our study opens novel insights to help understand the mechanisms of lncRNA RHPN1-AS1/miR-485-5p/BSG axis in HCC progression, which may provide a new therapeutic target for HCC treatment.
据报道，长链非编码RNA RHPN1-AS1（lncRNA RHPN1-AS1）在多种癌症中发挥癌基因的作用；然而，lncRNA RHPN1-AS1在肝细胞癌（HCC）中的作用尚待研究。这项工作的主要目的是研究lncRNA RHPN1-AS1/miR-485-5p系统对HCC细胞增殖、迁移和侵袭的影响，并进一步探究其潜在机制。我们的研究发现，lncRNA RHPN1-AS1在HCC组织和细胞系中显著上调，尤其是在HCCLM3和SMMC-7721细胞中。敲低lncRNA RHPN1-AS1可降低HCCLM3和SMMC-7721细胞的增殖、侵袭和迁移能力，而miR-485-5p抑制剂可削弱这种作用。此外，lncRNA RHPN1-AS1沉默后，基底膜蛋白（BSG）的表达降低，表明lncRNA RHPN1-AS1/miR-485-5p/BSG轴在HCC进展中发挥作用。我们的研究为理解lncRNA RHPN1-AS1/miR-485-5p/BSG轴在HCC进展中的机制提供了新的见解，这可能为HCC治疗提供新的治疗靶点。
