Department of Gynaecology and Obstetrics, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, Shandong 264100, P.R. China.
Oncol Rep. 2021 Jun;45(6). doi: 10.3892/or.2021.8062. Epub 2021 Apr 28.
Long non‑coding RNAs (lncRNAs) play a crucial role in cancer development. However, researchers have yet to identify the underlying association between lncRNAs and ovarian cancer (OC). The aim of the present study was to examine the effect of lncRNA RHPN1‑AS1 (RHPN1‑AS1) on OC cells and tissues. Reverse transcriptase‑quantitative PCR (RT‑qPCR) was utilized to quantify RHPN1‑AS1, miR‑485‑5p, and mRNA expression in samples with OC. Luciferase‑reporter assay, RNA immunoprecipitation (RIP) assay, and RNA pull‑down assay were then employed to validate the target relationship among RHPN1‑AS1, miR‑485‑5p and . Cell Counting Kit‑8, BrdU, wound‑healing, cell‑adhesion, and flow cytometry assays were also employed to assess cell viability, proliferation, migration, adhesion and apoptosis, respectively, in SKOV3 and OVCAR3 cell lines. Findings revealed that RHPN1‑AS1 demonstrated a higher expression level in OC cell lines and tissues. In addition, RHPN1‑AS1 enhanced the adhesion, proliferation and migration of OC cell lines but decreased apoptosis of OC cells. It was also observed that the relationship between RHPN1‑AS1 and miR‑485‑5p was negative and that RHPN1‑AS1 could sponge miR‑485‑5p to regulate the proliferation, apoptosis, adhesion, and migration abilities of OC cells. Moreover, was targeted by miR‑485‑5p and was significantly overexpressed in OC cell lines and tissues. Experimental investigations also revealed that promoted the proliferation, adhesion, and migration of OC cells but suppressed the apoptosis of SKOV3 and OVCAR3 cells. In summary, RHPN1‑AS1 played a tumor promotive role by sponging miR‑485‑5p to increase expression in OC tumorigenesis.
长链非编码 RNA（lncRNA）在癌症发展中发挥着关键作用。然而，研究人员尚未确定 lncRNA 与卵巢癌（OC）之间的潜在关联。本研究旨在研究 lncRNA RHPN1-AS1（RHPN1-AS1）对 OC 细胞和组织的影响。逆转录-定量 PCR（RT-qPCR）用于定量 OC 样本中 RHPN1-AS1、miR-485-5p 和 mRNA 的表达。然后使用荧光素酶报告基因检测、RNA 免疫沉淀（RIP）测定和 RNA 下拉测定来验证 RHPN1-AS1、miR-485-5p 和 之间的靶标关系。细胞计数试剂盒-8（CCK-8）、BrdU、划痕愈合、细胞黏附以及流式细胞术实验也用于评估 SKOV3 和 OVCAR3 细胞系中的细胞活力、增殖、迁移、黏附和凋亡。结果表明，RHPN1-AS1 在 OC 细胞系和组织中表达水平较高。此外，RHPN1-AS1 增强了 OC 细胞系的黏附、增殖和迁移，但降低了 OC 细胞的凋亡。还观察到 RHPN1-AS1 与 miR-485-5p 呈负相关，并且 RHPN1-AS1 可以吸附 miR-485-5p 来调节 OC 细胞的增殖、凋亡、黏附和迁移能力。此外， 是 miR-485-5p 的靶标，在 OC 细胞系和组织中显著过表达。实验研究还表明， 促进了 OC 细胞的增殖、黏附和迁移，但抑制了 SKOV3 和 OVCAR3 细胞的凋亡。综上所述，RHPN1-AS1 通过吸附 miR-485-5p 增加 OC 肿瘤发生中的 表达，发挥肿瘤促进作用。
