Heydari Shabnam, Pirzad Alireza
Department of Plant Production and Genetics, Faculty of Agriculture and Natural Resources, Urmia University, Urmia, Iran.
Curr Microbiol. 2020 Sep;77(9):2523-2534. doi: 10.1007/s00284-020-02034-y. Epub 2020 May 20.
Salinity, a serious environmental pressure on crop production, might be counteracted by free-living and symbiotic inoculants entailing positive synergistic effects. Enhancement in nutrient uptake and/or production of antioxidants under the stress condition, can improve plant growth and yield. In this study, inoculation of Lallemantia iberica with Funneliformis mosseae and the sulfur solubilizing bacterium (Thiobacillus sp. T95 and T40) was evaluated under two salinity levels (6.72 dS/m and 0.91 dS/m as control). The root colonization, spore density, seed and biological yield, total soluble sugars, and nutrients were reduced by salt stress. Antioxidant enzyme activity (catalase, superoxide dismutase, peroxidase and ascorbate peroxidase), proline, contents of sodium and sulfur have increased under salt stress. The enzyme activities as well as the concentrations of nitrogen, phosphorus, potassium, sodium, and sulfur were dropped at the flowering stage (75 days after sowing). Seed and biological yield, antioxidant enzymes activity, proline content, and nutrients were significantly improved in mycorrhizal treatments. Inoculation of Thiobacillus exhibited the positive effect on root colonization, spore density, enzymes activity, and nutrients. Bacterial treatments (dual and single) significantly increased the sulfur and total soluble sugars. Totally, the mycorrhizal plants accumulated more enzymatically produced antioxidants, osmolytes, and showed improved nutrient uptake. Our results provide new insights into the relationship among arbuscular mycorrhizal fungi (AMF), biosulfur bacteria, and plant growth under saline conditions. In conclusion, the Lallemantia iberica inoculation with mycorrhizal fungi, either alone, or in combination with Thiobacillus, is indicated for optimum plant yield through alleviation of the salinity stress.
盐分是影响作物产量的严重环境压力因素，而自由生活和共生的接种剂可能会对其产生抵消作用，并带来积极的协同效应。在胁迫条件下提高养分吸收和/或抗氧化剂的产生，可以促进植物生长并提高产量。在本研究中，在两种盐分水平（6.72 dS/m和作为对照的0.91 dS/m）下，评估了用摩西斗管囊霉和硫氧化细菌（硫杆菌属T95和T40）接种伊朗琉璃苣的效果。盐胁迫降低了根定殖、孢子密度、种子和生物产量、总可溶性糖以及养分含量。盐胁迫下抗氧化酶活性（过氧化氢酶、超氧化物歧化酶、过氧化物酶和抗坏血酸过氧化物酶）、脯氨酸、钠和硫的含量增加。在开花期（播种后75天），酶活性以及氮、磷、钾、钠和硫的浓度下降。菌根处理显著提高了种子和生物产量、抗氧化酶活性、脯氨酸含量和养分含量。接种硫杆菌对根定殖、孢子密度、酶活性和养分有积极影响。细菌处理（双重和单一）显著增加了硫和总可溶性糖。总体而言，菌根植物积累了更多酶促产生的抗氧化剂和渗透调节物质，并表现出更好的养分吸收。我们的研究结果为盐胁迫条件下丛枝菌根真菌（AMF）、生物硫细菌与植物生长之间的关系提供了新的见解。总之，接种菌根真菌单独或与硫杆菌联合接种伊朗琉璃苣，可通过缓解盐胁迫实现最佳植物产量。
