Munyemana Jean Claude, Chen Jia, Wei Xin, Ali Mohammad Chand, Han Yangxia, Qiu Hongdeng
CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, Gansu, China.
University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing, 100039, China.
Anal Bioanal Chem. 2020 Jul;412(19):4629-4638. doi: 10.1007/s00216-020-02712-7. Epub 2020 May 21.
In the quest for alternative products that would conquer natural enzyme drawbacks, enzyme-like nanomaterials with controllable morphology, high catalytic activity, excellent stability, and reusability have gained extensive attention in recent years. Herein, a simple and versatile strategy based on basic deep eutectic solvents was used to create layered copper hydroxide nitrate (Cu(OH)NO) with a well-structured nanosheet-like morphology. The present nanosheets exhibited extraordinary oxidase and peroxidase-like activity. More importantly, these nanosheets have shown the ability to operate at low and high temperatures with appreciable stability and multiple reusabilities. Based on inhibiting the oxidase-like activity of the prepared Cu(OH)NO, we designed a colorimetric sensing technique with a high-efficiency detection of biothiols in serum samples. Because of the simplicity and low-cost fabrication approach, our findings would be beneficial to the artificial enzyme research community as another facile and green tactics to fabricate heterogeneous artificial enzymes. Graphical abstract.
在寻求克服天然酶缺点的替代产品的过程中，近年来，具有可控形态、高催化活性、出色稳定性和可重复使用性的类酶纳米材料受到了广泛关注。在此，基于碱性深共熔溶剂的一种简单通用策略被用于制备具有结构良好的纳米片状形态的层状碱式硝酸铜（Cu(OH)NO）。所制备的纳米片表现出非凡的氧化酶和过氧化物酶样活性。更重要的是，这些纳米片已显示出在低温和高温下具有可观稳定性和多次重复使用性的操作能力。基于抑制所制备的Cu(OH)NO的氧化酶样活性，我们设计了一种比色传感技术，用于高效检测血清样品中的生物硫醇。由于制备方法简单且成本低，我们的研究结果作为另一种简便且绿色的制备多相人工酶的策略，将对人工酶研究领域有益。图形摘要。
