Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Henan Normal University, Xinxiang, Henan 453007, PR China; Henan Key Laboratory of Green Chemical Media and Reactions, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, Henan 453007, PR China.
Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Henan Normal University, Xinxiang, Henan 453007, PR China; Henan Key Laboratory of Green Chemical Media and Reactions, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, Henan 453007, PR China.
Anal Chim Acta. 2017 Jan 25;951:124-132. doi: 10.1016/j.aca.2016.11.035. Epub 2016 Nov 25.
The synthesized NiCoO mesoporous spheres (MS) displayed intrinsic peroxidase and oxidase-like activity were firstly reported. The catalytic mechanism of the oxidase-like activity of NiCoO MS was analyzed in detail using the electron spin resonance (ESR) method. It is found that NiCoO MS could directly oxidize 3,3',5,5'-tetramethylbenzidine (TMB) but did not produce O and ·OH. And the mechanism of the peroxidase-like activity of NiCoO MS was also verified that the oxidation of TMB stemmed from not only ·OH but also O. Based on the NiCoO MS showed excellent peroxidase-like activity over a broad temperature range, especially at normal body temperature, a detection tool was designed for glucose determination in diabetics' serum samples. And this detection method based on NiCoO MS gave a lower limit of detection than the method using CoO NPs and NiO NPs, as the single-component oxides of NiCoO. Our study may open up the possibility to make a great influence on the next generation of enzyme mimetics system.
首次报道了具有内源性过氧化物酶和氧化酶样活性的合成 NiCoO 介孔球 (MS)。详细分析了 NiCoO MS 氧化酶样活性的催化机制，采用电子顺磁共振 (ESR) 方法。结果发现，NiCoO MS 可直接氧化 3,3',5,5'-四甲基联苯胺 (TMB)，但不产生 O 和·OH。并验证了 NiCoO MS 过氧化物酶样活性的机制，TMB 的氧化不仅源于·OH，还源于 O。基于 NiCoO MS 在较宽的温度范围内表现出优异的过氧化物酶样活性，尤其是在正常体温下，设计了一种用于检测糖尿病患者血清样品中葡萄糖的检测工具。与使用 CoO NPs 和 NiO NPs 的方法相比，这种基于 NiCoO MS 的检测方法具有更低的检测限，因为 NiCoO 是 NiCoO 的单一成分氧化物。我们的研究可能为下一代酶模拟系统产生重大影响开辟了可能性。
