Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, People's Republic of China.
Institute of Plant Nutrition and Resources, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.
Environ Sci Pollut Res Int. 2020 Aug;27(23):29257-29265. doi: 10.1007/s11356-020-08902-1. Epub 2020 May 21.
Pot experiments were set up to simulate the soil contamination by three initial concentrations of sulfamethoxazole (SMX) (S1, 100 mg/kg; S2, 200 mg/kg; S3, 300 mg/kg). The content of SMX in soil and its accumulation in lettuce were analysed. Additionally, the effects of SMX on soil microorganisms and antibiotic resistance genes were studied by Illumina high-throughput sequencing and droplet digital polymerase chain reaction (ddPCR). The results demonstrated that the SMX content in soil reduced by 97%, 86% and 75% in the S1, S2 and S3 treatment groups after 120 days, respectively. The accumulated SMX in lettuce was positively correlated with the initial concentration of SMX in soil. SMX contamination significantly reduced the bacterial diversity and altered the composition of bacterial and fungal communities in soil. The dominant bacterial and fungal genera in the SMX-contaminated soil were obviously different from those in the control soil. The relative abundance of sul1 (sulfonamide resistance gene) remarkably increased in the SMX-contaminated soil, while that of other ARGs, such as sul2 and tetracycline and quinolone resistance genes, showed no significant change.
设置了盆栽实验来模拟土壤受三种初始磺胺甲恶唑（SMX）浓度（S1，100mg/kg；S2，200mg/kg；S3，300mg/kg）污染的情况。分析了土壤中 SMX 的含量及其在生菜中的积累情况。此外，通过 Illumina 高通量测序和液滴数字聚合酶链反应（ddPCR）研究了 SMX 对土壤微生物和抗生素抗性基因的影响。结果表明，在 120 天后，S1、S2 和 S3 处理组土壤中 SMX 的含量分别减少了 97%、86%和 75%。生菜中积累的 SMX 与土壤中 SMX 的初始浓度呈正相关。SMX 污染显著降低了土壤中细菌的多样性，并改变了土壤中细菌和真菌群落的组成。受 SMX 污染的土壤中的优势细菌和真菌属明显不同于对照土壤中的优势细菌和真菌属。受 SMX 污染的土壤中 sul1（磺胺类抗性基因）的相对丰度显著增加，而其他 ARGs（如 sul2 和四环素及喹诺酮类抗性基因）的相对丰度没有明显变化。
