Armstrong D W, Faulkner J R, Han S M
Department of Chemistry, University of Missouri-Rolla 65401-0249.
J Chromatogr. 1988 Oct 28;452:323-30. doi: 10.1016/s0021-9673(01)81457-6.
Partially substituted hydroxypropyl-beta-cyclodextrin and hydroxyethyl-beta-cyclodextrin proved to be effective chiral mobile phase additives (CMAs) for the thin-layer chromatographic (TLC) resolution of racemic benzyl-2-oxazolidinone, 5-(4-methyl-phenyl)-5-phenylhydantoin, mephenytoin and several dansyl and beta-naphthylamide amino acids. Several diastereomeric compounds including steroid epimers and alkaloids were separated as well. The derivatized beta-cyclodextrins tended to be much more soluble in water and hydro-organic solvents than native beta-cyclodextrin. Their chromatographic selectivity also was somewhat different. The use of CMAs in TLC is a potentially useful and powerful method that has not been considered adequately. The relative lack of chiral stationary phases available in planar format makes the use of CMAs particularly attractive.
部分取代的羟丙基-β-环糊精和羟乙基-β-环糊精被证明是有效的手性流动相添加剂（CMA），可用于外消旋苄基-2-恶唑烷酮、5-（4-甲基苯基）-5-苯基乙内酰脲、美芬妥因以及几种丹磺酰和β-萘酰胺氨基酸的薄层色谱（TLC）拆分。几种非对映异构体化合物，包括甾体差向异构体和生物碱，也得到了分离。衍生化的β-环糊精在水和水-有机溶剂中的溶解度往往比天然β-环糊精大得多。它们的色谱选择性也有所不同。在TLC中使用CMA是一种潜在有用且强大的方法，但尚未得到充分考虑。平面形式的手性固定相相对较少，这使得使用CMA特别具有吸引力。
