Calumpang Carla Lenore F, Saigo Tomoki, Watanabe Mutsumi, Tohge Takayuki
Graduate School of Science and Technology, Nara Institute of Science and Technology (NAIST), Ikoma, Nara 630-0192, Japan.
Metabolites. 2020 May 19;10(5):209. doi: 10.3390/metabo10050209.
Many solanaceous crops are an important part of the human daily diet. Fruit polyphenolics are plant specialized metabolites that are recognized for their human health benefits and their defensive role against plant abiotic and biotic stressors. Flavonoids and chlorogenates are the major polyphenolic compounds found in solanaceous fruits that vary in quantity, physiological function, and structural diversity among and within plant species. Despite their biological significance, the elucidation of metabolic shifts of polyphenols during fruit ripening in different fruit tissues, has not yet been well-characterized in solanaceous crops, especially at a cross-species and cross-cultivar level. Here, we performed a cross-species comparison of fruit-metabolomics to elucidate the metabolic regulation of fruit polyphenolics from three representative crops of Solanaceae (tomato, eggplant, and pepper), and a cross-cultivar comparison among different pepper cultivars ( cv.) using liquid chromatography-mass spectrometry (LC-MS). We observed a metabolic trade-off between hydroxycinnamates and flavonoids in pungent pepper and anthocyanin-type pepper cultivars and identified metabolic signatures of fruit polyphenolics in each species from each different tissue-type and fruit ripening stage. Our results provide additional information for metabolomics-assisted crop improvement of solanaceous fruits towards their improved nutritive properties and enhanced stress tolerance.
许多茄科作物是人类日常饮食的重要组成部分。果实多酚类物质是植物特化代谢产物，因其对人类健康有益以及对植物非生物和生物胁迫的防御作用而受到认可。黄酮类化合物和绿原酸是茄科果实中发现的主要多酚类化合物，它们在植物物种之间和内部的数量、生理功能和结构多样性方面存在差异。尽管它们具有生物学意义，但在茄科作物中，不同果实组织在果实成熟过程中多酚代谢变化的阐明尚未得到很好的表征，尤其是在跨物种和跨品种水平上。在这里，我们进行了果实代谢组学的跨物种比较，以阐明茄科三种代表性作物（番茄、茄子和辣椒）果实多酚的代谢调控，并使用液相色谱 - 质谱联用（LC-MS）对不同辣椒品种（cv.）进行了跨品种比较。我们观察到辛辣辣椒和花青素型辣椒品种中羟基肉桂酸酯和黄酮类化合物之间的代谢权衡，并确定了来自每种不同组织类型和果实成熟阶段的每个物种中果实多酚的代谢特征。我们的结果为代谢组学辅助茄科果实改良提供了额外信息，以提高其营养特性和增强胁迫耐受性。
