Baglivo Ilaria, Pirone Luciano, Malgieri Gaetano, Fattorusso Roberto, Roop Ii Roy Martin, Pedone Emilia Maria, Pedone Paolo Vincenzo
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies University of Campania 'Luigi Vanvitelli' Caserta Italy.
Institute of Biostructures and Bioimaging C.N.R. Naples Italy.
FEBS Open Bio. 2018 Mar 31;8(4):711-718. doi: 10.1002/2211-5463.12411. eCollection 2018 Apr.
The protein MucR from spp. is involved in the expression regulation of genes necessary for host interaction and infection. MucR is a member of the Ros/MucR family, which comprises prokaryotic zinc-finger proteins and includes Ros from and the Ml proteins from . MucR from spp. can regulate the expression of virulence genes and repress its own gene expression. Despite the well-known role played by MucR in the repression of its own gene, no target sequence has yet been identified in the promoter gene. In this study, we provide the first evidence that MucR from binds more than one target site in the promoter region of its own gene, suggesting a molecular mechanism by which this protein represses its own expression. Furthermore, a circular dichroism analysis reveals that MucR is a heat-stable protein. Overall, the results of this study suggest that MucR might resemble a H-NS protein.
来自某菌属的蛋白质MucR参与宿主相互作用和感染所需基因的表达调控。MucR是Ros/MucR家族的成员，该家族由原核锌指蛋白组成，包括来自某菌的Ros和来自某菌的Ml蛋白。来自某菌属的MucR可以调节毒力基因的表达并抑制其自身基因的表达。尽管MucR在抑制自身基因方面所起的作用已广为人知，但在其启动子基因中尚未鉴定出靶序列。在本研究中，我们首次提供证据表明来自某菌的MucR在其自身基因的启动子区域结合多个靶位点，这表明了该蛋白抑制自身表达的分子机制。此外，圆二色性分析表明MucR是一种热稳定蛋白。总体而言，本研究结果表明MucR可能类似于一种H-NS蛋白。
